Receptors for insulin-like growth factors I and II: autoradiographic localization in rat brain and comparison to receptors for insulin

Lesniak, M A; Hill, J M; Kiess, W; Rojeski, M; Pert, C B; Roth, J

doi:10.1210/endo-123-4-2089

Title: Receptors for insulin-like growth factors I and II: autoradiographic localization in rat brain and comparison to receptors for insulin

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Abstract

Receptors for insulin-like growth factor I (IGF-I) in rat brain were visualized using autoradiography with (125I)IGF-I. The binding of the labeled peptide was competed for fully by high concentrations of unlabeled IGF-I. At intermediate concentrations of unlabeled peptide the binding of (125I)IGF-I was competed for by unlabeled IGF-I more effectively than by IGF-II or insulin, which is typical of receptors for IGF-I. Essentially every brain section shows specific binding of IGF-I, and the pattern of binding of IGF-I to its receptors correlated well with the cytoarchitectonic structures. In parallel studies we showed that (125I)IGF-II was bound to tissue sections of rat brain and that the binding was competed for by an excess of unlabeled IGF-II. However, intermediate concentrations of unlabeled peptides gave inconclusive results. To confirm that the binding of (125I)IGF-II was to IGF-II receptors, we showed that antibodies specific for the IGF-II receptor inhibited the binding of labeled IGF-II. Furthermore, the binding of the antibody to regions of the brain section, visualized by the application of (125I)protein-A, gave patterns indistinguishable from those obtained with (125I)IGF-II alone. Again, the binding was very widely distributed throughout the central nervous system, and the patterns of distribution corresponded well to the underlying neuralmore »« less

Authors:: Lesniak, M A; Hill, J M; Kiess, W; Rojeski, M; Pert, C B; Roth, J

Publication Date:: Sat Oct 01 00:00:00 EDT 1988

Research Org.:: National Institute of Diabetes, Digestive and Kidney Diseases, Bethesda, MD (USA)

OSTI Identifier:: 7095803

Resource Type:: Journal Article

Journal Name:: Endocrinology; (United States)

Additional Journal Information:: Journal Volume: 123:4

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; GROWTH FACTORS; RECEPTORS; INSULIN; BIOLOGICAL LOCALIZATION; ANTIBODIES; AUTORADIOGRAPHY; BIOCHEMICAL REACTION KINETICS; BRAIN; COMPARATIVE EVALUATIONS; INHIBITION; IODINE 125; RATS; SPECIFICITY; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CENTRAL NERVOUS SYSTEM; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPES; KINETICS; MAMMALS; MEMBRANE PROTEINS; MITOGENS; NERVOUS SYSTEM; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats


                    Lesniak, M A, Hill, J M, Kiess, W, Rojeski, M, Pert, C B, and Roth, J. Receptors for insulin-like growth factors I and II: autoradiographic localization in rat brain and comparison to receptors for insulin.  United States: N. p., 1988. 
        Web.  doi:10.1210/endo-123-4-2089.

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                    Lesniak, M A, Hill, J M, Kiess, W, Rojeski, M, Pert, C B, & Roth, J. Receptors for insulin-like growth factors I and II: autoradiographic localization in rat brain and comparison to receptors for insulin.  United States.  https://doi.org/10.1210/endo-123-4-2089

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                    Lesniak, M A, Hill, J M, Kiess, W, Rojeski, M, Pert, C B, and Roth, J. 1988.  
        "Receptors for insulin-like growth factors I and II: autoradiographic localization in rat brain and comparison to receptors for insulin".  United States.  https://doi.org/10.1210/endo-123-4-2089.

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@article{osti_7095803,

  title        = {Receptors for insulin-like growth factors I and II: autoradiographic localization in rat brain and comparison to receptors for insulin},

  author       = {Lesniak, M A and Hill, J M and Kiess, W and Rojeski, M and Pert, C B and Roth, J},

  abstractNote = {Receptors for insulin-like growth factor I (IGF-I) in rat brain were visualized using autoradiography with (125I)IGF-I. The binding of the labeled peptide was competed for fully by high concentrations of unlabeled IGF-I. At intermediate concentrations of unlabeled peptide the binding of (125I)IGF-I was competed for by unlabeled IGF-I more effectively than by IGF-II or insulin, which is typical of receptors for IGF-I. Essentially every brain section shows specific binding of IGF-I, and the pattern of binding of IGF-I to its receptors correlated well with the cytoarchitectonic structures. In parallel studies we showed that (125I)IGF-II was bound to tissue sections of rat brain and that the binding was competed for by an excess of unlabeled IGF-II. However, intermediate concentrations of unlabeled peptides gave inconclusive results. To confirm that the binding of (125I)IGF-II was to IGF-II receptors, we showed that antibodies specific for the IGF-II receptor inhibited the binding of labeled IGF-II. Furthermore, the binding of the antibody to regions of the brain section, visualized by the application of (125I)protein-A, gave patterns indistinguishable from those obtained with (125I)IGF-II alone. Again, the binding was very widely distributed throughout the central nervous system, and the patterns of distribution corresponded well to the underlying neural structures. Densitometric analysis of the receptors enabled us to compare the distribution of IGF-I receptors with that of IGF-II receptors as well as retrospectively with that of insulin receptors.},

  doi          = {10.1210/endo-123-4-2089},

  url          = {https://www.osti.gov/biblio/7095803},
  journal      = {Endocrinology; (United States)},
number       = ,

  volume       = 123:4,

  place        = {United States},

  year         = {Sat Oct 01 00:00:00 EDT 1988},

  month        = {Sat Oct 01 00:00:00 EDT 1988}

}

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