Nonimmunogenic radiation-induced lymphoma: immunity induction by a somatic cell hybrid

Journal Article · Sat May 01 00:00:00 EDT 1982 · J. Natl. Cancer Inst.; (United States)

OSTI ID:7088734

Yefenof, E; Goldapfel, M; Ber, R

The cell line designated PIR-2 is a nonimmunogenic X-ray-induced thymoma of C57BL/6 origin that is unable to induce antitumor immunity in syngeneic lymphocytes in vitro and in mice in vivo. Fusion of PIR-2 with an allogeneic universal fuser A9HT (clone 3c) resulted in the establishment of a somatic cell hybrid designated A9/PIR. C57BL/6 lymphocytes sensitized in vitro with A9/PIR could lyse parental PIR-2 cells, as well as other syngeneic tumors. However, immunization of mice with the hybrid significantly enhanced PIR-2 tumor takes while it partially protected the animals against a challenge with unrelated syngeneic tumors. The results imply that somatic cell hybridization can increase the immunogenicity of an otherwise nonimmunogenic tumor. However, in view of the enhancing effects of hybrid preimmunization on parental tumor cell growth, the possible application of this approach for immunotherapy is questionable.

• Research Organization: The Hebrew University-Hadassah Medical School, Jerusalem, Israel
• OSTI ID: 7088734
• Journal Information: J. Natl. Cancer Inst.; (United States), Vol. 68:5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYBRIDIZATION
IMMUNOTHERAPY
LYMPHOMAS
RADIOINDUCTION
IMMUNITY
LYMPHOCYTES
X RADIATION
ANIMAL CELLS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
IONIZING RADIATIONS
LEUKOCYTES
MATERIALS
NEOPLASMS
RADIATIONS
SOMATIC CELLS
THERAPY
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551000 - Physiological Systems
560121 - Radiation Effects on Cells- External Source- (-1987)