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Title: Nonimmunogenic radiation-induced lymphoma: immunity induction by a somatic cell hybrid

The cell line designated PIR-2 is a nonimmunogenic X-ray-induced thymoma of C57BL/6 origin that is unable to induce antitumor immunity in syngeneic lymphocytes in vitro and in mice in vivo. Fusion of PIR-2 with an allogeneic universal fuser A9HT (clone 3c) resulted in the establishment of a somatic cell hybrid designated A9/PIR. C57BL/6 lymphocytes sensitized in vitro with A9/PIR could lyse parental PIR-2 cells, as well as other syngeneic tumors. However, immunization of mice with the hybrid significantly enhanced PIR-2 tumor takes while it partially protected the animals against a challenge with unrelated syngeneic tumors. The results imply that somatic cell hybridization can increase the immunogenicity of an otherwise nonimmunogenic tumor. However, in view of the enhancing effects of hybrid preimmunization on parental tumor cell growth, the possible application of this approach for immunotherapy is questionable.
Authors:
; ;
Publication Date:
OSTI Identifier:
7088734
Resource Type:
Journal Article
Resource Relation:
Journal Name: J. Natl. Cancer Inst.; (United States); Journal Volume: 68:5
Research Org:
The Hebrew University-Hadassah Medical School, Jerusalem, Israel
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; HYBRIDIZATION; IMMUNOTHERAPY; LYMPHOMAS; RADIOINDUCTION; IMMUNITY; LYMPHOCYTES; X RADIATION; ANIMAL CELLS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DISEASES; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; LEUKOCYTES; MATERIALS; NEOPLASMS; RADIATIONS; SOMATIC CELLS; THERAPY 550602* -- Medicine-- External Radiation in Diagnostics-- (1980-); 551000 -- Physiological Systems; 560121 -- Radiation Effects on Cells-- External Source-- (-1987)