Inhibition of semiconservative DNA synthesis in ICR 2A frog cells exposed to monochromatic uv wavelengths (252-313 nm) and photoreactivating light
Exposure of ICR 2A frog cells to monochromatic uv wavelengths in the range 252-313 nm caused an inhibition of semiconservative DNA synthesis which was partially relieved in cells receiving a post irradiation treatment with photoreactivating light (>350 nm). Hence pyrimidine dimers acted as lesions blocking DNA synthesis in uv-irradiated cells based upon the specificity of photoreactivating enzyme for the light-dependent monomerization of dimers in DNA. Compared with the shorter wavelengths tested, however, this recovery of DNA synthesis was not as great in cells exposed to 302-nm radiation and was nearly absent in 313-nm-irradiated cells up to 12 hr after treatment. These results suggest that nondimer photoproducts also play an important role in causing DNA synthesis inhibition in cells exposed to wavelengths greater than 300 nm.
- Research Organization:
- Univ. of Texas Health Science Center, Dallas
- OSTI ID:
- 7086102
- Journal Information:
- Radiat. Res.; (United States), Vol. 90:3
- Country of Publication:
- United States
- Language:
- English
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Inhibition of semiconservative DNA synthesis in ICR 2A frog cells by pyrimidine dimers and nondimer photoproducts induced by ultraviolet radiation
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Related Subjects
ANIMAL CELLS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
INHIBITION
PHOTOREACTIVATION
DNA
DIMERS
FROGS
MONOCHROMATIC RADIATION
ULTRAVIOLET RADIATION
AMPHIBIANS
ANIMALS
AQUATIC ORGANISMS
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
ELECTROMAGNETIC RADIATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RECOVERY
REPAIR
SYNTHESIS
VERTEBRATES
560121* - Radiation Effects on Cells- External Source- (-1987)
560114 - Radiation Effects on Biochemicals- In Animals- (-1987)