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Title: Contribution of depressed reuptake to the depletion of norepinephrine from rat heart and spleen during endotoxin shock

Abstract

Norepinephrine content (microgram/g) was depressed in hearts and spleens of fasted male Holtzman rats treated intravenously with Salmonella enteritidis lipopolysaccharide (14-17 mg/kg). To investigate the mechanism of norepinephrine depletion during endotoxicosis, in vivo norepinephrine reuptake was evaluated in control and severely shocked rats using the incorporation of /sup 3/H-norepinephrine into hearts and spleens. Incorporation of /sup 3/H-norepinephrine into spleens of endotoxic rats was reduced 88%. In contrast, cardiac tissue incorporation of /sup 3/H-norepinephrine was not significantly impaired. In vitro analysis of total norepinephrine retained in cardiac and splenic tissue slices incubated with /sup 3/H-norepinephrine yielded results consistent with in vivo experiments: Splenic norepinephrine reuptake was significantly decreased on the order of 50% in preparations from endotoxic rats, while myocardial norepinephrine reuptake was the same in both groups. The results indicate that depression of norepinephrine reuptake is a mechanism of norepinephrine depletion in spleens but not hearts of endotoxic rats.

Authors:
; ;
Publication Date:
Research Org.:
Department of Physiology, Loyola University of Chicago, Stritch School of Medicine, Maywood, Illinois
OSTI Identifier:
7071739
Resource Type:
Journal Article
Journal Name:
Circ. Shock; (United States)
Additional Journal Information:
Journal Volume: 9:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ADRENALINE; QUANTITATIVE CHEMICAL ANALYSIS; RATS; BIOLOGICAL SHOCK; COMPARATIVE EVALUATIONS; HEART; PHYSIOLOGY; SALMONELLA; SPLEEN; TOXICITY; TRITIUM COMPOUNDS; ADRENAL HORMONES; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BACTERIA; BODY; CARDIOTONICS; CARDIOVASCULAR AGENTS; CARDIOVASCULAR SYSTEM; CHEMICAL ANALYSIS; DRUGS; HORMONES; LABELLED COMPOUNDS; MAMMALS; MICROORGANISMS; NEUROREGULATORS; ORGANS; PATHOLOGICAL CHANGES; RODENTS; STEROID HORMONES; SYMPATHOMIMETICS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques; 400102 - Chemical & Spectral Procedures

Citation Formats

Pardini, B J, Jones, S B, and Filkins, J P. Contribution of depressed reuptake to the depletion of norepinephrine from rat heart and spleen during endotoxin shock. United States: N. p., 1982. Web.
Pardini, B J, Jones, S B, & Filkins, J P. Contribution of depressed reuptake to the depletion of norepinephrine from rat heart and spleen during endotoxin shock. United States.
Pardini, B J, Jones, S B, and Filkins, J P. 1982. "Contribution of depressed reuptake to the depletion of norepinephrine from rat heart and spleen during endotoxin shock". United States.
@article{osti_7071739,
title = {Contribution of depressed reuptake to the depletion of norepinephrine from rat heart and spleen during endotoxin shock},
author = {Pardini, B J and Jones, S B and Filkins, J P},
abstractNote = {Norepinephrine content (microgram/g) was depressed in hearts and spleens of fasted male Holtzman rats treated intravenously with Salmonella enteritidis lipopolysaccharide (14-17 mg/kg). To investigate the mechanism of norepinephrine depletion during endotoxicosis, in vivo norepinephrine reuptake was evaluated in control and severely shocked rats using the incorporation of /sup 3/H-norepinephrine into hearts and spleens. Incorporation of /sup 3/H-norepinephrine into spleens of endotoxic rats was reduced 88%. In contrast, cardiac tissue incorporation of /sup 3/H-norepinephrine was not significantly impaired. In vitro analysis of total norepinephrine retained in cardiac and splenic tissue slices incubated with /sup 3/H-norepinephrine yielded results consistent with in vivo experiments: Splenic norepinephrine reuptake was significantly decreased on the order of 50% in preparations from endotoxic rats, while myocardial norepinephrine reuptake was the same in both groups. The results indicate that depression of norepinephrine reuptake is a mechanism of norepinephrine depletion in spleens but not hearts of endotoxic rats.},
doi = {},
url = {https://www.osti.gov/biblio/7071739}, journal = {Circ. Shock; (United States)},
number = ,
volume = 9:2,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1982},
month = {Fri Jan 01 00:00:00 EST 1982}
}