Platelet-derived growth factor stimulated mechanisms of glucosamine incorporation

Journal Article · Thu Oct 01 00:00:00 EDT 1987 · American Journal of Physiology; (USA)

OSTI ID:7068554

Harrington, M A; Pledger, W J ^[1]

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1. Univ. of North Carolina, Chapel Hill (USA)

Platelet-derived growth factor (PDGF) treatment of density-arrested BALB/c-3T3 cells results in increased ({sup 3}H)glucosamine (GlcN) incorporation into cellular material. The enhanced GlcN incorporation is not due to a preferential increase in proteoglycan synthesis as measured by ({sup 35}S)H{sub 2}SO{sub 4} incorporation. Approximately 50% of the GlcN incorporated in PDGF or platelet-poor plasma (PPP)-treated cultures enters N-linked glycoproteins. Addition of dolichol-phosphate (dolichol-P), a required intermediate in N-linked glycosylation, did not alter ({sup 3}H)GlcN incorporation in PDGF-treated cells but did increase incorporation in PPP-treated cultures to a level comparable to that observed for PDGF-treated cultures. PDGF-treated cultures contained twofold greater quantities of ({sup 3}H)GlcN dolichol intermediates and lipid-free glycoprotein. Over a 12-h time course 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG CoA reductase) activity was similar in cultures treated with PDGF or PPP. Results of these studies reveal that enhanced protein glycosylation in response to PDGF treatment is not the result of a direct effect on HMG CoA reductase.

• OSTI ID: 7068554
• Journal Information: American Journal of Physiology; (USA), Vol. 253:4; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSAMINE
UPTAKE
GROWTH FACTORS
BIOLOGICAL EFFECTS
TRITIUM COMPOUNDS
BLOOD PLATELETS
CELL CYCLE
COENZYMES
FIBROBLASTS
OXIDOREDUCTASES
SULFUR 35
SULFURIC ACID
AMINES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ENZYMES
EVEN-ODD NUCLEI
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
INORGANIC ACIDS
ISOTOPES
LIGHT NUCLEI
MATERIALS
MITOGENS
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SULFUR ISOTOPES
550201* - Biochemistry- Tracer Techniques