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Title: Engraftment of DLA-nonidentical unrelated canine marrow after high-dose fractionated total body irradiation

Marrow transplants were carried out between unrelated DLA-nonidentical dogs. Recipients were conditioned for transplantation by total body irradiation (TBI) given either as a single dose of 9 Gy (900 rad) or fractionated in three increments of 6 Gy (600 rad) each at intervals of 48 hr. All recipients received marrow, less than or equal to to 4 X 10/sup 8/ cells/kg, and no buffy coat cells. No immunosuppression was given after grafting. All 10 dogs given single-dose total body irradiation failed to show engraftment and died with marrow aplasia and infectious complications (median survival 12 days). In contrast, all 10 dogs given fractionated TBI had sustained engraftment and died with graft-versus-host disease (GVHD) and infectious complications (median survival 12.5 days). None of the dogs died from radiation-induced gastroenteritis.In conclusion, resistance to DLA-nonidentical unrelated marrow grafts can be abrogated by high-dose TBI. This technique may allow hemopoietic engraftment even after in vitro manipulation of the marrow such as lymphocyte depletion by cell separation or treatment with anti-T cell antisera.
Authors:
 [1] ; ; ; ; ;
  1. (Fred Hutchinson Cancer Research Center, Seattle, WA)
Publication Date:
OSTI Identifier:
7066015
Resource Type:
Journal Article
Resource Relation:
Journal Name: Transplantation; (United States); Journal Volume: 33:4
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; BONE MARROW; TRANSPLANTS; IMMUNITY; BIOLOGICAL RADIATION EFFECTS; IMMUNOSUPPRESSION; RADIOINDUCTION; BLOOD COUNT; COBALT 60; DOGS; FRACTIONATED IRRADIATION; GAMMA RADIATION; GRAFT-HOST REACTION; INFECTIOUS DISEASES; WHOLE-BODY IRRADIATION; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BODY; COBALT ISOTOPES; DISEASES; ELECTROMAGNETIC RADIATION; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; IONIZING RADIATIONS; IRRADIATION; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; MAMMALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANS; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; TISSUES; VERTEBRATES; YEARS LIVING RADIOISOTOPES 560152* -- Radiation Effects on Animals-- Animals; 551000 -- Physiological Systems