Angiotensin converting enzyme in the brain, testis, epididymis, pituitary gland and adrenal gland

Thesis/Dissertation · Wed Jan 01 00:00:00 EST 1986

OSTI ID:7064757

Strittmatter, S M

(/sup 3/H)Captopril binds to angiotensin converting enzyme (ACE) in rat tissue homogenates. The pharmacology, regional distribution and copurification of (/sup 3/H)captopril binding with enzymatic activity demonstrate the selectivity of (/sup 3/H)captopril labeling of ACE. (/sup 3/H)Captopril binding to purified ACE reveals differences in cationic dependence and anionic regulation between substrate catalysis and inhibitor recognition. (/sup 3/H)Captopril association with ACE is entropically driven. The selectivity of (/sup 3/H)captopril binding permits autoradiographic localization of the ACE in the brain, male reproductive system, pituitary gland and adrenal gland. In the brain, ACE is visualized in a striatonigral neuronal pathway which develops between 1 and 7 d after birth. In the male reproductive system, (/sup 3/H)captopril associated silver grains are found over spermatid heads and in the lumen of seminiferous tubules in stages I-VIII and XII-XIV. In the pituitary gland, ACE is localized to the posterior lobe and patches of the anterior lobe. The adrenal medulla contains moderate ACE levels while low levels are found in the adrenal cortex. Adrenal medullary ACE is increased after hypophysectomy and after reserpine treatment. The general of ligand binding techniques for the study of enzymes is demonstrated by the specific labeling of another enzyme, enkephaline convertase, in crude tissue homogenates by the inhibitor (/sup 3/H)GEMSA.

• Research Organization: Johns Hopkins Univ., Baltimore, MD (USA)
• OSTI ID: 7064757
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
59 BASIC BIOLOGICAL SCIENCES
BRAIN
AUTORADIOGRAPHY
PEPTIDE HYDROLASES
TISSUE DISTRIBUTION
PITUITARY GLAND
TESTES
ADRENAL GLANDS
ANGIOTENSIN
PHARMACOLOGY
RATS
TRITIUM COMPOUNDS
ANIMALS
BODY
CARDIOVASCULAR AGENTS
CENTRAL NERVOUS SYSTEM
DISTRIBUTION
DRUGS
ENDOCRINE GLANDS
ENZYMES
GLANDS
GLOBULINS
GONADS
HYDROLASES
LABELLED COMPOUNDS
MALE GENITALS
MAMMALS
NERVOUS SYSTEM
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
VASOCONSTRICTORS
VERTEBRATES
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