Deuterium nuclear magnetic resonance study of protein dynamics and protein-lipid interactions in model and biological membranes

Thesis/Dissertation · Sun Jan 01 00:00:00 EST 1984

OSTI ID:7063601

Kintanar, A B

The dynamics of the Halobacterium halobium purple membrane protein, bacteriorhodopsin; and the motional order of the fluorescent lipid probe, diphenylhexatriene, in various model membrane systems, have been investigated by deuterium nuclear magnetic resonance (NMR) spectroscopy. /sup 2/H NMR spectra and spin-lattice relaxation times of selectively-deuterated amino acids in the crystalline solid state and in biosynthetically enriched bacteriorhodopsin, have been obtained. Analysis of the data has led to the determination of the type and rate of amino acid motion in these systems. The motions of amino acid residues, are shown to be very sensitive to packing considerations. Large differences in both the type and rate of motion are observed for a particular amino acid in various crystal lattice forms and in the membrane protein. Moreover, motional heterogeneity is found to occur in some crystal lattice forms and especially in bacteriorhodopsin. The major types of motion that are observed include methyl group rotation (by three-fold hops), phenyl ring flips (180/sup 0/ hops), ring libration, chain libration, side-chain hops, and isotropic reorientation. The last two motions are seen only in the membrane protein. All of these motions can occur at widely different rates depending on the packing environment, but in bacteriorhodopsin, these rates are generally greater than 10/sup 6/ sec/sup -1/. /sup 2/H NMR spectra of selectively-deuterated diphenylhexatriene in dimyristoylphosphatidylcholine multilamellar bilayers in the liquid crystalline phase, as a function of temperature and membrane composition, have also been obtained.

• Research Organization: Illinois Univ., Urbana (USA)
• OSTI ID: 7063601
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LIPIDS
CONFIGURATION INTERACTION
PHOTOSYNTHETIC BACTERIA
CELL MEMBRANES
PROTEINS
DEUTERIUM
FLUORESCENCE
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEAR MAGNETIC RESONANCE
RHODOPSIN
CELL CONSTITUENTS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
LUMINESCENCE
MAGNETIC RESONANCE
MEMBRANES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PIGMENTS
RESONANCE
STABLE ISOTOPES
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