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Title: In vivo traffic of indium-111-oxine labeled human lymphocytes collected by automated apheresis

Abstract

The in vivo traffic patterns of autologous lymphocytes were studied in five normal human volunteers using lymphocytes obtained by automated apheresis, separated on Ficoll-Hypaque gradients, and labeled ex vivo with {sup 111}In-oxine. Final lymphocyte infusions contained 1.8-3.1 X 10(9) cells and 270-390 microCi (9.99-14.43 MBq) {sup 111}In, or 11-17 microCi (0.41-0.63 MBq) per 10(8) lymphocytes. Gamma imaging showed transient lung uptake and significant retention of radioactivity in the liver and spleen. Progressive uptake of activity in normal, nonpalpable axillary and inguinal lymph nodes was seen from 24 to 96 hr. Accumulation of radioactivity also was demonstrated at the forearm skin test site, as well as in its associated epitrochlear and axillary lymph nodes, in a subject who had been tested for delayed hypersensitivity with tetanus toxoid. Indium-111-oxine labeled human lymphocytes may provide a useful tool for future studies of normal and abnormal lymphocyte traffic.

Authors:
; ; ; ;  [1]
  1. National Institutes of Health, Bethesda, MD (USA)
Publication Date:
OSTI Identifier:
7063384
Resource Type:
Journal Article
Journal Name:
Journal of Nuclear Medicine; (USA)
Additional Journal Information:
Journal Volume: 31:6; Journal ID: ISSN 0161-5505
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; LYMPHOCYTES; LABELLING; BIOLOGICAL LOCALIZATION; IMAGE PROCESSING; INDIUM 111; LIVER; LUNGS; LYMPH NODES; MAN; OXINE; SPLEEN; ANIMAL CELLS; ANIMALS; AROMATICS; AZAARENES; AZINES; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; ELECTRON CAPTURE RADIOISOTOPES; GLANDS; HETEROCYCLIC COMPOUNDS; HYDROXY COMPOUNDS; INDIUM ISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; LEUKOCYTES; LYMPHATIC SYSTEM; MAMMALS; MATERIALS; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PRIMATES; PROCESSING; PYRIDINES; QUINOLINES; RADIOISOTOPES; RESPIRATORY SYSTEM; SOMATIC CELLS; VERTEBRATES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Read, E J, Keenan, A M, Carter, C S, Yolles, P S, and Davey, R J. In vivo traffic of indium-111-oxine labeled human lymphocytes collected by automated apheresis. United States: N. p., 1990. Web.
Read, E J, Keenan, A M, Carter, C S, Yolles, P S, & Davey, R J. In vivo traffic of indium-111-oxine labeled human lymphocytes collected by automated apheresis. United States.
Read, E J, Keenan, A M, Carter, C S, Yolles, P S, and Davey, R J. 1990. "In vivo traffic of indium-111-oxine labeled human lymphocytes collected by automated apheresis". United States.
@article{osti_7063384,
title = {In vivo traffic of indium-111-oxine labeled human lymphocytes collected by automated apheresis},
author = {Read, E J and Keenan, A M and Carter, C S and Yolles, P S and Davey, R J},
abstractNote = {The in vivo traffic patterns of autologous lymphocytes were studied in five normal human volunteers using lymphocytes obtained by automated apheresis, separated on Ficoll-Hypaque gradients, and labeled ex vivo with {sup 111}In-oxine. Final lymphocyte infusions contained 1.8-3.1 X 10(9) cells and 270-390 microCi (9.99-14.43 MBq) {sup 111}In, or 11-17 microCi (0.41-0.63 MBq) per 10(8) lymphocytes. Gamma imaging showed transient lung uptake and significant retention of radioactivity in the liver and spleen. Progressive uptake of activity in normal, nonpalpable axillary and inguinal lymph nodes was seen from 24 to 96 hr. Accumulation of radioactivity also was demonstrated at the forearm skin test site, as well as in its associated epitrochlear and axillary lymph nodes, in a subject who had been tested for delayed hypersensitivity with tetanus toxoid. Indium-111-oxine labeled human lymphocytes may provide a useful tool for future studies of normal and abnormal lymphocyte traffic.},
doi = {},
url = {https://www.osti.gov/biblio/7063384}, journal = {Journal of Nuclear Medicine; (USA)},
issn = {0161-5505},
number = ,
volume = 31:6,
place = {United States},
year = {Fri Jun 01 00:00:00 EDT 1990},
month = {Fri Jun 01 00:00:00 EDT 1990}
}