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Title: Toxicity of metallic ions and oxides to rabbit alveolar macrophages

The effects of soluble compounds and oxides of As, Cd, Cu, Hg, Ni, Pb, Sb, Sn, V, and Zn on oxidative metabolism and membrane integrity of rabbit alveolar macrophages were studied by 24-hr in vitro exposure. Oxidative metabolism induced by phagocytosis of opsonized zymosan was measured by H{sub 2}O{sub 2} and O{sub 2}{sup {minus}} release and by chemiluminescence in the presence of luminol. Membrane integrity was estimated by extracellular LDH activity. Metallic ions and oxides inhibited the release of active oxygen species. Cd(II), As(III), and V(V) were the most toxic elements as measured by all investigated parameters. Cu(II) decreased O{sub 2}{sup {minus}} release and chemiluminescence effectively but H{sub 2}O{sub 2} release and membrane integrity less. Chemiluminescence was decreased strongly by Hg(II) while O{sub 2}{sup {minus}} and H{sub 2}O{sub 2} release were depressed moderately. Zn(II) and Sb(III) compounds caused medium toxicity and the tested Sn, Ni, and Pb compounds showed only faint toxic effects.
Authors:
; ; ;  [1]
  1. (Univ. of Hamburg (Germany, F.R.))
Publication Date:
OSTI Identifier:
7059152
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environmental Research; (USA); Journal Volume: 48:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANTIMONY; TOXICITY; ARSENIC COMPOUNDS; CADMIUM COMPOUNDS; COPPER COMPOUNDS; LEAD COMPOUNDS; MACROPHAGES; INJURIES; MERCURY COMPOUNDS; NICKEL COMPOUNDS; VANADIUM; ZINC; CELL MEMBRANES; CHEMILUMINESCENCE; COMPARATIVE EVALUATIONS; HYDROGEN PEROXIDE; LUNGS; METABOLISM; OXYGEN; RADICALS; ANIMAL CELLS; BODY; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; ELEMENTS; HYDROGEN COMPOUNDS; LUMINESCENCE; MEMBRANES; METALS; NONMETALS; ORGANS; OXYGEN COMPOUNDS; PEROXIDES; PHAGOCYTES; RESPIRATORY SYSTEM; SOMATIC CELLS; TRANSITION ELEMENT COMPOUNDS; TRANSITION ELEMENTS 560300* -- Chemicals Metabolism & Toxicology