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Title: Investigation of laser-induced retinal damage. Annual report, 1 Apr 91-31 Mar 92

Abstract

Laser-induced, photooxidative damage in ocular tissue was studied with a quantitative assay using high performance liquid chromatography (HPLC) to separate oxidized and reduced ascorbic acid in exposed tissue components. We demonstrated that ascorbic acid, incubated with whole, bovine retinal pigment epithelial (RPE) cells, was oxidized when the reaction mixture was exposed to the output of an argon-ion continuous wave laser The amount of ascorbic acid oxidized was proportional to the irradiance of the sample, and the reaction was wavelength-dependent, with short-wavelength visible light more effective than long-wavelengths in driving the, reaction. The photosensitizing activity was associated with the RPE melanin pigment granules, and was not lost after disrupting or heating the RPE cells. Because melanin was known to form free radicals when illuminated, we hypothesized that ascorbic acid detoxified the light-activated melanin free radicals while being itself oxidized in process. If the supply of reduced ascorbic acid were exhausted, however, the activated melanin could have been the source of tissue-damaging radicals. This model was consistent with a photochemical damage mechanism involving light-activated melanin.

Authors:
;
Publication Date:
Research Org.:
Texas Univ., San Antonio, TX (United States). Health Science Center
OSTI Identifier:
7049858
Report Number(s):
AD-A-250173/2/XAB
CNN: AFOSR-91-0208
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ASCORBIC ACID; REDOX REACTIONS; LASER RADIATION; BIOLOGICAL EFFECTS; RETINA; SENSITIVITY; ANTIOXIDANTS; ARGON; GAS LASERS; LIQUID COLUMN CHROMATOGRAPHY; MELANIN; RADICALS; BODY; BODY AREAS; CHEMICAL REACTIONS; CHROMATOGRAPHY; ELECTROMAGNETIC RADIATION; ELEMENTS; EYES; FACE; FLUIDS; GASES; HEAD; HYDROXY COMPOUNDS; LASERS; NONMETALS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PIGMENTS; RADIATIONS; RARE GASES; SENSE ORGANS; SEPARATION PROCESSES; VITAMINS; 560400* - Other Environmental Pollutant Effects

Citation Formats

Glickman, R D, and Lam, K W. Investigation of laser-induced retinal damage. Annual report, 1 Apr 91-31 Mar 92. United States: N. p., 1992. Web.
Glickman, R D, & Lam, K W. Investigation of laser-induced retinal damage. Annual report, 1 Apr 91-31 Mar 92. United States.
Glickman, R D, and Lam, K W. 1992. "Investigation of laser-induced retinal damage. Annual report, 1 Apr 91-31 Mar 92". United States.
@article{osti_7049858,
title = {Investigation of laser-induced retinal damage. Annual report, 1 Apr 91-31 Mar 92},
author = {Glickman, R D and Lam, K W},
abstractNote = {Laser-induced, photooxidative damage in ocular tissue was studied with a quantitative assay using high performance liquid chromatography (HPLC) to separate oxidized and reduced ascorbic acid in exposed tissue components. We demonstrated that ascorbic acid, incubated with whole, bovine retinal pigment epithelial (RPE) cells, was oxidized when the reaction mixture was exposed to the output of an argon-ion continuous wave laser The amount of ascorbic acid oxidized was proportional to the irradiance of the sample, and the reaction was wavelength-dependent, with short-wavelength visible light more effective than long-wavelengths in driving the, reaction. The photosensitizing activity was associated with the RPE melanin pigment granules, and was not lost after disrupting or heating the RPE cells. Because melanin was known to form free radicals when illuminated, we hypothesized that ascorbic acid detoxified the light-activated melanin free radicals while being itself oxidized in process. If the supply of reduced ascorbic acid were exhausted, however, the activated melanin could have been the source of tissue-damaging radicals. This model was consistent with a photochemical damage mechanism involving light-activated melanin.},
doi = {},
url = {https://www.osti.gov/biblio/7049858}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Apr 22 00:00:00 EDT 1992},
month = {Wed Apr 22 00:00:00 EDT 1992}
}

Technical Report:
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