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Title: Relative rates of albumin equilibration in the skin interstitium and lymph during increased permeability

Abstract

The initial equilibration of /sup 125/I-labelled albumin between the vascular and extravascular compartments was studied in hindpaw heel skin of anesthetized rabbits. Bradykinin (0.3 ..mu..g/min) was infused into a small branch of the femoral artery. A second group of rabbits served as control. Following bradykinin, prenodal popliteal lymph flow was 4 times control flow. The lymph-to-plasma concentration ratios for total protein and albumin were, respectively, 60% and 50% larger than control. Tissue albumin concentration was twice control. After reaching a steady, elevated lymph flow, tracer albumin was infused to maintain plasma activity constant for 3 hrs. The plasma volume in tissue samples was measured using /sup 131/I-labeled albumin injected 10 min before ending the experiment. Endogenous albumin was measured in plasma, lymph, and tissue samples using rocket electroimmunoassay. After 3 hrs of tracer infusion, lymph specific activity was 3 times greater than control. In the control group, plasma albumin equilibrated more rapidly with lymph than with tissue (p < 0.05). Following bradykinin, extravascular specific activity was 4 times control, resulting in lymph and tissue equilibrating with plasma at similar rates. Thus, increasing capillary permeability causes the extravascular albumin mass to behave as if distributed in a single compartment.

Authors:
; ;
Publication Date:
Research Org.:
Albany Medical College, Albany, NY
OSTI Identifier:
7035333
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 87-010334
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ALBUMINS; TISSUE DISTRIBUTION; CAPILLARIES; PERMEABILITY; BLOOD PLASMA; BRADYKININ; FLUID FLOW; IODINE 125; IODINE 131; LYMPH; RABBITS; SKIN; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD VESSELS; BODY; BODY FLUIDS; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KININS; MAMMALS; MATERIALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOISOTOPES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Powers, M R, Wallace, J R, and Bell, D R. Relative rates of albumin equilibration in the skin interstitium and lymph during increased permeability. United States: N. p., 1986. Web.
Powers, M R, Wallace, J R, & Bell, D R. Relative rates of albumin equilibration in the skin interstitium and lymph during increased permeability. United States.
Powers, M R, Wallace, J R, and Bell, D R. 1986. "Relative rates of albumin equilibration in the skin interstitium and lymph during increased permeability". United States.
@article{osti_7035333,
title = {Relative rates of albumin equilibration in the skin interstitium and lymph during increased permeability},
author = {Powers, M R and Wallace, J R and Bell, D R},
abstractNote = {The initial equilibration of /sup 125/I-labelled albumin between the vascular and extravascular compartments was studied in hindpaw heel skin of anesthetized rabbits. Bradykinin (0.3 ..mu..g/min) was infused into a small branch of the femoral artery. A second group of rabbits served as control. Following bradykinin, prenodal popliteal lymph flow was 4 times control flow. The lymph-to-plasma concentration ratios for total protein and albumin were, respectively, 60% and 50% larger than control. Tissue albumin concentration was twice control. After reaching a steady, elevated lymph flow, tracer albumin was infused to maintain plasma activity constant for 3 hrs. The plasma volume in tissue samples was measured using /sup 131/I-labeled albumin injected 10 min before ending the experiment. Endogenous albumin was measured in plasma, lymph, and tissue samples using rocket electroimmunoassay. After 3 hrs of tracer infusion, lymph specific activity was 3 times greater than control. In the control group, plasma albumin equilibrated more rapidly with lymph than with tissue (p < 0.05). Following bradykinin, extravascular specific activity was 4 times control, resulting in lymph and tissue equilibrating with plasma at similar rates. Thus, increasing capillary permeability causes the extravascular albumin mass to behave as if distributed in a single compartment.},
doi = {},
url = {https://www.osti.gov/biblio/7035333}, journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:4,
place = {United States},
year = {Wed Mar 05 00:00:00 EST 1986},
month = {Wed Mar 05 00:00:00 EST 1986}
}

Conference:
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