Characterization of bromine-77-labeled proteins prepared using bromoperoxidase

Conference · Wed Oct 01 00:00:00 EDT 1980 · J. Nucl. Med.; (United States)

OSTI ID:7031742

McElvany, K D; Welch, M J

The halogenating enzyme bromoperoxidase, isolated from the red algae Bonnemaisonia hamifera and Penicillus capitatus, was used to catalyze the radiohalogenation of proteins at neutral pH. Human serum albumin and canine fibrinogen were halogenated as model compounds; the proteins were labeled with Br-77, produced by the /sup 75/As(..cap alpha..,2n)/sup 77/Br reaction. For each enzyme, the essential reaction parameters (including the concentrations of hydrogen peroxide or of protein, the amount of enzyme used to catalyze the reaction, the pH of the reaction mixture, and the reaction time) were varied to obtain conditions that resulted in the highest yield of radiolabeled protein. The labeled proteins prepared with bromoperoxidase are stable with respect to loss of the radiolabel by hydrolysis and retain their biologic activity. The extension of this method to radiobromination of other types of compounds for imaging and receptor studies seems promising.

• Research Organization: Washington Univ., St. Louis, MO
• OSTI ID: 7031742
• Journal Information: J. Nucl. Med.; (United States), Vol. 21:10; Conference: 28. annual meeting of the Society of Nuclear Medicine, Las Vegas, NV, USA, 16 Jun 1981
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BROMINE 77
COMPARATIVE EVALUATIONS
SCINTISCANNING
IODINE 125
LABELLED COMPOUNDS
CHEMICAL PREPARATION
THROMBOSIS
TRACER TECHNIQUES
ALBUMINS
DOGS
FIBRINOGEN
MAN
ORGANIC BROMINE COMPOUNDS
PEROXIDASES
PH VALUE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BLOOD COAGULATION FACTORS
BROMINE ISOTOPES
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLOBULINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PORPHYRINS
PRIMATES
PROTEINS
RADIOISOTOPE SCANNING
RADIOISOTOPES
SYNTHESIS
VASCULAR DISEASES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
560172 - Radiation Effects- Nuclide Kinetics & Toxicology- Animals- (-1987)