Assignment of the human ZNF83 (HPF1) zinc finger gene to chromosome 19q13. 3-q13. 4
- Universite de Liege, Sart-Tilman (Belgium)
- School of Medicine, New Haven, CT (United States)
- Universite Libre de Bruxelles, Rhode-St-Genese (Belgium)
The authors have isolated a collection of human ZFPs encoding cDNAs (HPF1 to -9) by hybridization with a finger motif oligonucleotide probe. Here, they describe the localization of a chromosome 19-linked human ZFP gene (HPF1/ZNF83). They first assigned the ZNF83 gene on chromosome 19 by the screening of a human x rodent hybrid panel by DNA hybridization with a fragment of a previously cloned cDNA (data not shown). To further localize the gene within chromosome 19, the regional assignment of the ZNF83 gene was determined by fluorescence in situ hybridization and digital imaging microscopy as described elsewhere. Human metaphase spreads were hybridized with biotinylated ZNF83 cDNA, and hybridization was detected with fluorescein isothiocyanate-conjugated avidin-DCS. Chromosomes were identified by staining with 4,6-diamino-2-phenylindol dihydrochloride. The fractional length (Flpter) distance of the signal to the p arm terminus relative to the total chromosome length gave a Flpter value between 82.8 and 89.9, which is consistent with an assignment of the ZNF83 gene in ISCN region 19q13.3-q13.4. 14 refs., 1 fig.
- OSTI ID:
- 7029759
- Journal Information:
- Genomics; (United States), Vol. 21:1; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
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