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Title: Monoclonal antibodies against plant cell wall polysaccharides

Monoclonal antibodies (McAbs) are useful tools to probe the structure of plant cell wall polysaccharides and to localize these polysaccharides in plant cells and tissues. Murine McAbs were generated against the pectic polysaccharide, rhamnogalacturonan I (RG-I), isolated from suspension-cultured sycamore cells. The McAbs that were obtained were grouped into three classes based upon their reactivities with a variety of plant polysaccharides and membrane glycoproteins. Eleven McAbs (Class I) recognize epitope(s) that appear to be immunodominant and are found in RG-I from sycamore and maize, citrus pectin, polygalacturonic acid, and membrane glycoproteins from suspension-cultured cells of sycamore, maize, tobacco, parsley, and soybean. A second group of five McAbs (Class II) recognize epitope(s) present in sycamore RG-I, but do not bind to any of the other polysaccharides or glycoproteins recognized by Class I. Lastly, one McAb (Class III) reacts with sycamore RG-I, sycamore and tamarind xyloglucan, and sycamore and rice glucuronoarabinoxylan, but does not bind to maize RG-I, polygalacturonic acid or the plant membrane glycoproteins recognized by Class I. McAbs in Classes II and III are likely to be useful in studies of the structure, biosynthesis and localization of plant cell wall polysaccharides.
Authors:
; ; ;  [1]
  1. (Univ. of Georgia, Athens (USA))
Publication Date:
OSTI Identifier:
7025865
Resource Type:
Journal Article
Resource Relation:
Journal Name: Plant Physiology, Supplement; (USA); Journal Volume: 89:4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; POLYSACCHARIDES; BIOCHEMISTRY; CELL WALL; GLYCOPROTEINS; MONOCLONAL ANTIBODIES; PLANT CELLS; ANTIBODIES; CARBOHYDRATES; CELL CONSTITUENTS; CHEMISTRY; ORGANIC COMPOUNDS; PROTEINS; SACCHARIDES 551000* -- Physiological Systems