U.S. Department of EnergyOffice of Scientific and Technical Information
Permissive role of the acidification caused by wheat aleurone layers upon. alpha. -amylase induction by GA sub 3
Abstract
Wheat aleurone has the capacity of acidifying the incubation medium in 1 to 2 pH units. The {alpha}-amylase induction by GA{sub 3} in isolated wheat aleurone layers is strongly dependent on acidic pH of the medium (pH < 5). To examine possible mechanisms {sup 35}-Met incorporation into proteins and {alpha}-amylase, in the presence of GA{sub 3} and Ca{sup 2+} at pH, 4, 5 and 6 was studied. Although {sup 35}-Met uptake decreased markedly ({approx} 90%) at pH 4 in thepresence of GA{sub 3}, incorporation into total protein did not change significantly from other conditions. Auto-radiography of SDS-PAGE showed that most of the amino acid was in the {alpha}-amylase band, meaning that the effect of acidic pH is specific for GA{sub 3} actions on aleurone tissue. On the other hand, an increase of protonated GA{sub 3} diffusion could be ruled out. Also, there was not {alpha}-amylase inactivation at pH 6. These findings point out to the important physiological role of the acidification caused by the aleurone.
- Authors:
-
- CINVESTAV-IPN (Mexico)
- Publication Date:
- OSTI Identifier:
- 7025769
- Resource Type:
- Journal Article
- Journal Name:
- Plant Physiology, Supplement; (USA)
- Additional Journal Information:
- Journal Volume: 89:4; Journal ID: ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; WHEAT; PHYSIOLOGY; ACIDIFICATION; AMYLASE; AUTORADIOGRAPHY; ENZYME ACTIVITY; GIBBERELLIC ACID; HORMONES; LABELLED COMPOUNDS; METHIONINE; SULFUR 35; AMINO ACIDS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CEREALS; DAYS LIVING RADIOISOTOPES; DRUGS; ENZYMES; ESTERS; EVEN-ODD NUCLEI; GLYCOSYL HYDROLASES; GRASS; HETEROCYCLIC COMPOUNDS; HYDROLASES; HYDROXY ACIDS; ISOTOPES; LACTONES; LIGHT NUCLEI; LILIOPSIDA; LIPOTROPIC FACTORS; MAGNOLIOPHYTA; NUCLEI; O-GLYCOSYL HYDROLASES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; PLANTS; RADIOISOTOPES; SULFUR ISOTOPES; 551001* - Physiological Systems- Tracer Techniques
Citation Formats
Rodriguez-Campos, E, Bernal-Lugo, I, and Hamabata, A. Permissive role of the acidification caused by wheat aleurone layers upon. alpha. -amylase induction by GA sub 3. United States: N. p., 1989.
Web.
Rodriguez-Campos, E, Bernal-Lugo, I, & Hamabata, A. Permissive role of the acidification caused by wheat aleurone layers upon. alpha. -amylase induction by GA sub 3. United States.
Rodriguez-Campos, E, Bernal-Lugo, I, and Hamabata, A. 1989.
"Permissive role of the acidification caused by wheat aleurone layers upon. alpha. -amylase induction by GA sub 3". United States.
@article{osti_7025769,
title = {Permissive role of the acidification caused by wheat aleurone layers upon. alpha. -amylase induction by GA sub 3},
author = {Rodriguez-Campos, E and Bernal-Lugo, I and Hamabata, A},
abstractNote = {Wheat aleurone has the capacity of acidifying the incubation medium in 1 to 2 pH units. The {alpha}-amylase induction by GA{sub 3} in isolated wheat aleurone layers is strongly dependent on acidic pH of the medium (pH < 5). To examine possible mechanisms {sup 35}-Met incorporation into proteins and {alpha}-amylase, in the presence of GA{sub 3} and Ca{sup 2+} at pH, 4, 5 and 6 was studied. Although {sup 35}-Met uptake decreased markedly ({approx} 90%) at pH 4 in thepresence of GA{sub 3}, incorporation into total protein did not change significantly from other conditions. Auto-radiography of SDS-PAGE showed that most of the amino acid was in the {alpha}-amylase band, meaning that the effect of acidic pH is specific for GA{sub 3} actions on aleurone tissue. On the other hand, an increase of protonated GA{sub 3} diffusion could be ruled out. Also, there was not {alpha}-amylase inactivation at pH 6. These findings point out to the important physiological role of the acidification caused by the aleurone.},
doi = {},
url = {https://www.osti.gov/biblio/7025769},
journal = {Plant Physiology, Supplement; (USA)},
issn = {0079-2241},
number = ,
volume = 89:4,
place = {United States},
year = {Sat Apr 01 00:00:00 EST 1989},
month = {Sat Apr 01 00:00:00 EST 1989}
}
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