Immunologic and biochemical detection of cell surface alterations following morphological transformation or mutation of C3H/10T1/2 C1 8 cells by chemical carcinogens
The C3H/10T1/2 C1 in mouse embryo fibroblast cell line is a permanent, cultured line whose hypotetraploid cells are contact inhibited and nontumorigenic in immunosuppressed syngeneic mice. Upon treatment with certain chemical carcinogens or radiation, some of these cells become morphologically transformed so that they are no longer contact inhibited and form foci, which are tumorigenic in immunosuppressed, syngeneic mice. Resistance to the cardiac glycoside ouabain can also be chemically induced in these cells, presumably by mutation of the gene encoding the Na/sup +/, K/sup +/-dependent adenosinetriphosphatase cation transport enzyme. Thus C3H/10T1/2 C1 8 cells find utility as in vitro neoplastic transformation and mutation systems. Morphologic transformation induced by chemical carcinogens in C3H/10T1/2 cells is accompanied by complex antigenic changes. These changes were studied using /sup 125/I labelled Protein A IRMA and fluorescence-activated cell sorter immunofluorescence assays. A panel of thirty different polyclonal antisera was prepared against transformed and nontransformed C3H/10T1/2 cell lines. Mutation of the gene encoding the (Na,K)-ATPase enzyme is hypothesized to be responsible for the chemical carcinogen inducation of the ouabain resistance (Oua/sup r/) phenotype in C3H/10T1/2 cell lines. In order to test this hypothesis, the (Na,K)-ATPase enzyme activity of wild type and Oua/sup r/ C3H/10T1/2 cells were characterized for resistance to ouabain.
- Research Organization:
- University of Southern California, Los Angeles (USA)
- OSTI ID:
- 7023993
- Resource Relation:
- Other Information: Thesis
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
CARCINOGENS
BIOLOGICAL EFFECTS
EMBRYONIC CELLS
CARCINOGENESIS
ONCOGENIC TRANSFORMATIONS
RADIOIMMUNOASSAY
ATP-ASE
CELL MEMBRANES
ENZYME ACTIVITY
FIBROBLASTS
FLUORESCENCE
IODINE 125
MUTATIONS
OUABAIN
ACID ANHYDRASES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CARDIAC GLYCOSIDES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL TRANSFORMATIONS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLYCOSIDES
HYDROLASES
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LUMINESCENCE
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PHOSPHOHYDROLASES
RADIOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
SOMATIC CELLS
STROPHANTHINS
TRACER TECHNIQUES
550601* - Medicine- Unsealed Radionuclides in Diagnostics
560300 - Chemicals Metabolism & Toxicology