Separation, purification, and properties of cytochrome P-450 from uninduced rat liver microsomes for the studies of metabolism of environmental chemicals
- Tehran Univ. of Medical Sciences (Iran)
This study reports the authors present results on the development of a procedure for purification of multiple forms of cytochrome P-450 from un-induced rat liver microsomes. These cytochromes are catalytically active when reconstituted with NADPH-cytochrome c reductase and lipid and exhibit substrate specificities. The presence of four distinct forms of cytochrome P-450 in uninduced rat liver microsomes which is the result of this research report, compared with the presence of six forms in induced animals represent the importance of genetic control of these enzymes for the metabolism and detoxification of environmental chemicals. These metabolite patterns are not only different for the various species, but also among different individuals. The molecular basis for this are genetic and environmental factors, which exhibit interesting evolutionary aspects.
- OSTI ID:
- 7018258
- Report Number(s):
- CONF-8909236-; CODEN: ACWCA
- Journal Information:
- Preprints of Papers Presented at National Meeting, Division of Water, Air and Waste Chemistry, American Chemical Society; (USA), Vol. 28:2; Conference: JAERI/EPA workshop on residual radiation and recycle criteria, St. Michaels, MD (USA), 27-28 Sep 1989; ISSN 0099-7293
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MIXED-FUNCTION OXIDASES
CHEMICAL PROPERTIES
XENOBIOTICS
METABOLISM
CYTOCHROMES
FRACTIONATION
GENETIC VARIABILITY
LIVER
METABOLIC ACTIVATION
MICROSOMES
RATS
ANIMALS
BIOLOGICAL VARIABILITY
BODY
CELL CONSTITUENTS
DIGESTIVE SYSTEM
ENZYMES
GLANDS
MAMMALS
ORGANIC COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PIGMENTS
PROTEINS
RIBOSOMES
RODENTS
SEPARATION PROCESSES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology