Epidermal growth factor and its receptors in human pancreatic carcinoma

Journal Article · Tue May 01 00:00:00 EDT 1990 · Pancreas; (USA)

DOI:https://doi.org/10.1097/00006676-199005000-00006· OSTI ID:7014919

Chen, Y F; Pan, G Z; Hou, X; Liu, T H; Chen, J; Yanaihara, C; Yanaihara, N ^[1]

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1. Peking Union Medical College Hospital, Beijing (China)

The role of epidermal growth factor (EGF) in oncogenesis and progression of malignant tumors is a subject of vast interest. In this study, radioimmunoassay and radioreceptor assay of EGF were established. EGF contents in malignant and benign pancreatic tumors, in normal pancreas tissue, and in culture media of a human pancreatic carcinoma cell line were determined. EGF receptor binding studies were performed. It was shown that EGF contents in pancreatic carcinomas were significantly higher than those in normal pancreas or benign pancreatic tumors. EGF was also detected in the culture medium of a pancreatic carcinoma cell line. The binding of 125I-EGF to the pancreatic carcinoma cells was time and temperature dependent, reversible, competitive, and specific. Scatchard analysis showed that the dissociation constant of EGF receptor was 2.1 X 10(-9) M, number of binding sites was 1.3 X 10(5) cell. These results indicate that there is an over-expression of EGF/EGF receptors in pancreatic carcinomas, and that an autocrine regulatory mechanism may exist in the growth-promoting effect of EGF on tumor cells.

• OSTI ID: 7014919
• Journal Information: Pancreas; (USA), Vol. 5:3; ISSN 0885-3177
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GROWTH FACTORS
RECEPTORS
PANCREAS
CARCINOMAS
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL FUNCTIONS
EPIDERMIS
IODINE 125
MAN
ONCOGENIC TRANSFORMATIONS
RADIOIMMUNOASSAY
RADIORECEPTOR ASSAY
TEMPERATURE DEPENDENCE
TIME DEPENDENCE
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOASSAY
BODY
CELL TRANSFORMATIONS
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
ENDOCRINE GLANDS
EPITHELIUM
FUNCTIONS
GLANDS
IMMUNOASSAY
IMMUNOLOGY
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MITOGENS
NEOPLASMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RADIOISOTOPES
REACTION KINETICS
SKIN
TISSUES
TRACER TECHNIQUES
VERTEBRATES
550901* - Pathology- Tracer Techniques