The human enamel protein gene amelogenin is expressed from both the X and the Y chromosomes
- Faculty of Medicine, La Laguna (Spain)
- University of California School of Medicine, Torrence (United States)
- Lawrence Livermore National Laboratory, CA (United States)
Amelogenins, a family of extracellular matrix proteins of the dental enamel, are transiently but abundantly expressed by ameloblasts during tooth development. In this paper the authors report the characterization of the AMGX and AMGY genes on the short arms of the human X and Y chromosomes which encode the amelogenins. Their studies on the expression of the amelogenin genes in male developing tooth buds showed that both the AMGX and AMGY genes are transcriptionally active and encode potentially functional proteins. They have isolated genomic and cDNA clones form both the AMGX and AMGY loci and have studied the sequence organization of these two genes. Reverse transcriptase (RT)PCR amplification of the 5[prime] portion of the amelogenin transcripts revealed several alternatively spliced products. This information will be useful for studying the molecular basis of X-linked amelogenesis imperfecta, for understanding the evolution and regulation of gene expression on the mammalian sex chromosomes, and for investigating the role of amelogenin genes during tooth development.
- OSTI ID:
- 7014043
- Journal Information:
- American Journal of Human Genetics; (United States), Vol. 50:2; ISSN 0002-9297
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GENES
DNA SEQUENCING
HUMAN X CHROMOSOME
GENETIC MAPPING
HUMAN Y CHROMOSOME
BIOLOGICAL EVOLUTION
DENTIN
DNA POLYMERASES
GENE AMPLIFICATION
PROTEINS
RECOMBINANT DNA
TEETH
CHROMOSOMES
DIGESTIVE SYSTEM
DNA
ENZYMES
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
MAPPING
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORAL CAVITY
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
X CHROMOSOME
Y CHROMOSOME
550400* - Genetics