500-MHz proton NMR evidence for two solution structures of the common arm base-paired segment of wheat germ 5S ribosomal RNA
- Ohio State Univ., Columbus (USA)
The base-pair protons of the common arm duplex fragment of wheat germ (Triticum aestivum) ribosomal 5S RNA have been identified and assigned by means of 500-MHz proton NMR spectroscopy. The two previously reported extra base pairs within the fragment are now explained by the presence of two distinct solution structures of the common arm fragment (and its corresponding base-paired segment in intact 5S rRNA). The present conclusions are supported by one- and two-dimensional proton homonuclear Overhauser enhancements in H{sub 2}O and by temperature variation and Mg{sup 2+} titration of the downfield {sup 1}H NMR spectrum. The difference between the two conformers is most likely due to difference in helical tightness. Some additional amino proton resonances have also been assigned.
- OSTI ID:
- 7008927
- Journal Information:
- Biochemistry; (USA), Vol. 29:7; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
RIBOSOMAL RNA
NUCLEAR MAGNETIC RESONANCE
AQUEOUS SOLUTIONS
CATIONS
MAGNESIUM COMPOUNDS
OVERHAUSER EFFECT
PROTONS
WHEAT
ALKALINE EARTH METAL COMPOUNDS
BARYONS
CEREALS
CHARGED PARTICLES
DISPERSIONS
ELEMENTARY PARTICLES
FERMIONS
GRASS
HADRONS
IONS
LILIOPSIDA
MAGNETIC RESONANCE
MAGNOLIOPHYTA
MIXTURES
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
PLANTS
RESONANCE
RNA
SOLUTIONS
550601* - Medicine- Unsealed Radionuclides in Diagnostics