Aortic smooth muscle cell proteoglycan synthesis in relation to atherosclerosis

Miscellaneous · Sun Jan 01 00:00:00 EST 1989

OSTI ID:7008000

Edwards, I J

Proteoglycans (PG) are implicated in atherogenesis by their effects on tissue permeability and cell proliferation and their interaction with plasma low density lipoproteins. Using the pigeon model in which an atherosclerosis-susceptible (WC) and -resistant (SR) breed can be compared, PG synthesis by cultured aortic smooth muscle cells was examined by the use of ({sup 35}S)-sodium sulfate and ({sup 3}H)-serine or ({sup 3}H)-glucosamine as labeling precursors. In both SR and WC cells, the majority of newly synthesized PG were secreted into the media. Chondroitin sulfate (CS) PG and dermatan sulfate (DS) PG were the major PG produced. Total PG production was consistently lower in WC compared to SR cultures due in part to reduce PG synthesis but also to degradation of newly synthesized PG. Since increased DS-PG accompanines atherosclerosis progression, experiments were designed to test the hypothesis that macrophages modulate smooth muscle cell metabolism to cause increase DS-PG production. Cultured WC aortic smooth muscle cells were exposed to the media of cholesteryl ester-loaded pigeon peritoneal macrophages or a macrophage cell line P388D1 and the production of PG examined. Increasing concentration of conditioned media from both types of macrophages caused increased incorporation of {sup 35}S-sulfate into secreted PG, but no change in cell-associated PG. Lipopolysaccharide activation of P388D1 cells enhanced the effect.

• Research Organization: Wake Forest Univ., Winston-Salem, NC (USA)
• OSTI ID: 7008000
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ARTERIOSCLEROSIS
PATHOGENESIS
GLYCOPROTEINS
BIOSYNTHESIS
AORTA
BIRDS
GLUCOSAMINE
LIPOPOLYSACCHARIDES
MACROPHAGES
MUSCLES
PERMEABILITY
SERINE
SULFATES
SULFUR 35
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
AMINO ACIDS
ANIMAL CELLS
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BLOOD VESSELS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
EVEN-ODD NUCLEI
HEXOSAMINES
HEXOSES
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
LIPIDS
MONOSACCHARIDES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PHAGOCYTES
POLYSACCHARIDES
PROTEINS
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
SULFUR COMPOUNDS
SULFUR ISOTOPES
SYNTHESIS
VASCULAR DISEASES
VERTEBRATES
550901* - Pathology- Tracer Techniques