Phosphorylation potential and adenosine release during norepinephrine infusion in guinea pig heart

Journal Article · Sun Nov 01 00:00:00 EST 1987 · American Journal of Physiology; (USA)

OSTI ID:7007486

He, Miao-Xiang; Wangler, R D; Dillon, P F; Romig, G D; Sparks, H V ^[1]

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1. Michigan State Univ., East Lansing (USA)

This study tested the hypothesis that adenosine released from isolated guinea pig hearts in response to norepinephrine is related to the cellular phosphorylation potential (PP;(ATP)/(ADP)(P{sub i})), where P{sub i} is inorganic phosphate. {sup 31}P-nuclear magnetic resonance (NMR) was used to measure the relative concentrations of P{sub i}, phosphocreatine (PCr), and ATP. After a control period, norepinephrine was infused for 20 min during which {sup 31}P-NMR spectra and samples of venous effluent were collected every minute. With norepinephrine infusion, PCr decreased rapidly to 72% of control by 8 min and then recovered to 80% of control for the remaining 12 min. ATP fell slowly to 70% of control over 20 min. P{sub i} increased to a peak at 2 min, then declined slowly to a steady state from 8 to 20 min. Adenosine release increased at 7 min and then slowly fell to a steady state from 10 to 20 min. There is hyperbolic relationship between adenosine release and PP; when the PP declines, a level is reached below which there is a rapid increase in adenosine release. These data support the hypothesis that adenosine release is regulated by the cellular PP as a closely related variable.

• OSTI ID: 7007486
• Journal Information: American Journal of Physiology; (USA), Vol. 253:5; ISSN 0002-9513
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ADENOSINE
SECRETION
ADRENALINE
INFUSION
HEART
NUCLEAR MAGNETIC RESONANCE
ATP
BLOOD FLOW
GUINEA PIGS
NMR SPECTRA
PHOSPHOCREATINE
PHOSPHORUS 31
PHOSPHORYLATION
ADRENAL HORMONES
AMINO ACIDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOXYLIC ACIDS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
CHEMICAL REACTIONS
DRUGS
INTAKE
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
NEUROREGULATORS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
RESONANCE
RIBOSIDES
RODENTS
SPECTRA
STABLE ISOTOPES
SYMPATHOMIMETICS
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques