Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model
Abstract
The folding of chromatin in interphase cell nuclei was studied by fluorescent in situ hybridization with pairs of unique DNA sequence probes. The sites of DNA sequences separated by 100 to 2000 kilobase pairs (kbp) are distributed in interphase chromatin according to a random walk model. This model provides the basis for calculating the spacing of sequences along the linear DNA molecule from interphase distance measurements. An interphase mapping strategy based on this model was tested with 13 probes from a 4-megabase pair (Mbp) region of chromosome 4 containing the Huntington disease locus. The results confirmed the locations of the probes and showed that the remaining gap in the published maps of this region is negligible in size. Interphase distance measurements should facilitate construction of chromosome maps with an average marker density of one per 100 kbp, approximately ten times greater than that achieved by hybridization to metaphase chromosomes.
- Authors:
-
- Lawrence Livermore National Lab., Livermore, CA (United States)
- Univ. of California, Berkeley (United States)
- Publication Date:
- OSTI Identifier:
- 7007034
- Resource Type:
- Journal Article
- Journal Name:
- Science (Washington, D.C.); (United States)
- Additional Journal Information:
- Journal Volume: 257:5075; Journal ID: ISSN 0036-8075
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; CHROMATIN; GENETIC MAPPING; CELL CYCLE; CELL NUCLEI; CHROMOSOMES; DNA HYBRIDIZATION; DNA SEQUENCING; FLUORESCENCE; MATHEMATICAL MODELS; NERVOUS SYSTEM DISEASES; CELL CONSTITUENTS; DISEASES; HYBRIDIZATION; LUMINESCENCE; MAPPING; STRUCTURAL CHEMICAL ANALYSIS; 550400* - Genetics
Citation Formats
Engh, G van den, Trask, B J, and Sachs, R. Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model. United States: N. p., 1992.
Web. doi:10.1126/science.1388286.
Engh, G van den, Trask, B J, & Sachs, R. Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model. United States. https://doi.org/10.1126/science.1388286
Engh, G van den, Trask, B J, and Sachs, R. 1992.
"Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model". United States. https://doi.org/10.1126/science.1388286.
@article{osti_7007034,
title = {Estimating genomic distance from DNA sequence location in cell nuclei by a random walk model},
author = {Engh, G van den and Trask, B J and Sachs, R},
abstractNote = {The folding of chromatin in interphase cell nuclei was studied by fluorescent in situ hybridization with pairs of unique DNA sequence probes. The sites of DNA sequences separated by 100 to 2000 kilobase pairs (kbp) are distributed in interphase chromatin according to a random walk model. This model provides the basis for calculating the spacing of sequences along the linear DNA molecule from interphase distance measurements. An interphase mapping strategy based on this model was tested with 13 probes from a 4-megabase pair (Mbp) region of chromosome 4 containing the Huntington disease locus. The results confirmed the locations of the probes and showed that the remaining gap in the published maps of this region is negligible in size. Interphase distance measurements should facilitate construction of chromosome maps with an average marker density of one per 100 kbp, approximately ten times greater than that achieved by hybridization to metaphase chromosomes.},
doi = {10.1126/science.1388286},
url = {https://www.osti.gov/biblio/7007034},
journal = {Science (Washington, D.C.); (United States)},
issn = {0036-8075},
number = ,
volume = 257:5075,
place = {United States},
year = {Fri Sep 04 00:00:00 EDT 1992},
month = {Fri Sep 04 00:00:00 EDT 1992}
}