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Title: Evaluation of freeze fixation as a phytoplankton preservation method for microautoradiography

Abstract

Quantitative microautoradiography of marine and freshwater phytoplankton has been hampered by the fact that chemical techniques used to maintain structural integrity cause leakage of isotopically labeled cell constituents. Chemography, poor preservation of structural integrity, and leakage of cell constituents can all be avoided by quick-freezing filtered samples in liquid N/sub 2/ and then freeze-drying them before autoradiographic preparation. Leakage of fixed /sup 14/C and /sup 33/P and preservation of cell shapes and sizes by these preservation techniques are evaluated in diverse marine and freshwater phytoplankton communities.

Authors:
Publication Date:
Research Org.:
Univ. of North Carolina, Morehead City
OSTI Identifier:
7005874
Resource Type:
Journal Article
Journal Name:
Limnol. Oceanogr.; (United States)
Additional Journal Information:
Journal Volume: 29:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PHYTOPLANKTON; AUTORADIOGRAPHY; CYTOLOGICAL TECHNIQUES; CARBON 14; FREEZING; FRESH WATER; PHOSPHATES; PHOSPHORUS 33; SEAWATER; SODIUM CARBONATES; ALKALI METAL COMPOUNDS; AQUATIC ORGANISMS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON COMPOUNDS; CARBON ISOTOPES; CARBONATES; DAYS LIVING RADIOISOTOPES; EVEN-EVEN NUCLEI; HYDROGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; NUCLEI; ODD-EVEN NUCLEI; OXYGEN COMPOUNDS; PHOSPHORUS COMPOUNDS; PHOSPHORUS ISOTOPES; PLANKTON; RADIOISOTOPES; SODIUM COMPOUNDS; WATER; YEARS LIVING RADIOISOTOPES; 550701* - Microbiology- Tracer Techniques; 550301 - Cytology- Tracer Techniques

Citation Formats

Paerl, H W. Evaluation of freeze fixation as a phytoplankton preservation method for microautoradiography. United States: N. p., 1984. Web. doi:10.4319/lo.1984.29.2.0417.
Paerl, H W. Evaluation of freeze fixation as a phytoplankton preservation method for microautoradiography. United States. https://doi.org/10.4319/lo.1984.29.2.0417
Paerl, H W. 1984. "Evaluation of freeze fixation as a phytoplankton preservation method for microautoradiography". United States. https://doi.org/10.4319/lo.1984.29.2.0417.
@article{osti_7005874,
title = {Evaluation of freeze fixation as a phytoplankton preservation method for microautoradiography},
author = {Paerl, H W},
abstractNote = {Quantitative microautoradiography of marine and freshwater phytoplankton has been hampered by the fact that chemical techniques used to maintain structural integrity cause leakage of isotopically labeled cell constituents. Chemography, poor preservation of structural integrity, and leakage of cell constituents can all be avoided by quick-freezing filtered samples in liquid N/sub 2/ and then freeze-drying them before autoradiographic preparation. Leakage of fixed /sup 14/C and /sup 33/P and preservation of cell shapes and sizes by these preservation techniques are evaluated in diverse marine and freshwater phytoplankton communities.},
doi = {10.4319/lo.1984.29.2.0417},
url = {https://www.osti.gov/biblio/7005874}, journal = {Limnol. Oceanogr.; (United States)},
number = ,
volume = 29:2,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 1984},
month = {Thu Mar 01 00:00:00 EST 1984}
}