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Title: Extensive amino acid sequence homologies between animal lectins

The authors have established the amino acid sequence of the ..beta..-D-galactoside binding lectin from the electric eel and the sequences of several peptides from a similar lectin isolated from human placenta. These sequences were compared with the published sequences of peptides derived from the ..beta..-D-galactoside binding lectin from human lung and with sequences deduced from cDNAs assigned to the ..beta..-D-galactoside binding lectins from chicken embryo skin and human hepatomas. Significant homologies were observed. One of the highly conserved regions that contains a tryptophan residue and two glutamic acid resides is probably part of the ..beta..-D-galactoside binding site, which, on the basis of spectroscopic studies of the electric eel lectin, is expected to contain such residues. The similarity of the hydropathy profiles and the predicted secondary structure of the lectins from chicken skin and electric eel, in spite of differences in their amino acid sequences, strongly suggests that these proteins have maintained structural homologies during evolution and together with the other ..beta..-D-galactoside binding lectins were derived form a common ancestor gene.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
7000976
Resource Type:
Journal Article
Resource Relation:
Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States); Journal Volume: 84:18
Research Org:
University Paris, VIII (France)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; LECTINS; AMINO ACID SEQUENCE; BIOLOGICAL EVOLUTION; CHICKENS; EEL; ELECTROPHORESIS; EMBRYOS; GLUTAMIC ACID; HEPATOMAS; LIQUID COLUMN CHROMATOGRAPHY; MAN; PLACENTA; RECOMBINANT DNA; SKIN; TRYPTOPHAN; AMINO ACIDS; ANIMALS; AQUATIC ORGANISMS; AROMATICS; AZAARENES; AZOLES; BIRDS; BODY; CARBOXYLIC ACIDS; CHROMATOGRAPHY; DISEASES; DNA; FETAL MEMBRANES; FISHES; FOWL; HETEROCYCLIC ACIDS; HETEROCYCLIC COMPOUNDS; INDOLES; MAMMALS; MEMBRANES; MOLECULAR STRUCTURE; NEOPLASMS; NUCLEIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; PRIMATES; PYRROLES; SEPARATION PROCESSES; VERTEBRATES 500200* -- Environment, Atmospheric-- Chemicals Monitoring & Transport-- (-1989)