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Title: Fluorescence method for enzyme analysis which couples aromatic amines with aromatic aldehydes

Analysis of proteinases is accomplished using conventional amino acid containing aromatic amine substrates. Aromatic amines such as 4-methoxy-2-naphthylamine (4M2NA), 2-naphthylamine, aminoisophthalic acid dimethyl ester, p-nitroaniline, 4-methoxy-1-aminofluorene and coumarin derivatives resulting from enzymatic hydrolysis of the substrate couples with aromatic aldehydes such as 5-nitrosalicylaldehyde (5-NSA), benzaldehyde and p-nitrobenzaldehyde to produce Schiff-base complexes which are water insoluble. Certain Schiff-base complexes produce a shift from blue to orange-red (visible) fluorescence. Such complexes are useful in the assay of enzymes. No Drawings
Authors:
;
Publication Date:
OSTI Identifier:
7000909
Report Number(s):
US 4229528; A
PPN: US 5-959555
DOE Contract Number:
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 13 Nov 1978
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PEPTIDE HYDROLASES; BIOASSAY; CHEMICAL ANALYSIS; FLUORESCENCE; SUBSTRATES; ENZYMES; HYDROLASES; LUMINESCENCE; ORGANIC COMPOUNDS; PROTEINS 550200* -- Biochemistry