Protein, RNA, and DNA synthesis in cultures of skin fibroblasts from healthy subjects and patients with rheumatic diseases

Journal Article · Mon Jul 01 00:00:00 EDT 1985 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

DOI:https://doi.org/10.1007/BF00798662· OSTI ID:6999861

Abakumova, O Y; Kutsenko, N G; Panasyuk, A F

To study the mechanism of the lasting disturbance of fibroblast function, protein, RNA and DNA synthesis was investigated in skin fibroblasts from patients with rheumatoid arthritis (RA) and systemic scleroderma (SS). The labeled precursors used to analyze synthesis of protein, RNA, and DNA were /sup 14/C-protein hydrolysate, (/sup 14/C)uridine, and (/sup 14/C) thymidine. Stimulation was determined by measuring incorporation of (/sup 14/C)proline into fibroblast proteins. During analysis of stability of fast-labeled RNA tests were carried out to discover whether all measurable radioactivity belonged to RNA molecules.

• Research Organization: Institute of Biological and Medical Chemistry, Acad. of Sci., Moscow
• OSTI ID: 6999861
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 99:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
COLLAGEN
BIOSYNTHESIS
DNA
FIBROBLASTS
TRACER TECHNIQUES
RNA
SKIN
CARBON 14 COMPOUNDS
CELL CULTURES
LABELLING
PROLINE
RHEUMATIC DISEASES
SCINTILLATION COUNTING
SKIN DISEASES
THYMIDINE
AMINES
AMINO ACIDS
ANIMAL CELLS
AZINES
AZOLES
BODY
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
COUNTING TECHNIQUES
DISEASES
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRIMIDINES
PYRROLES
PYRROLIDINES
RIBOSIDES
SCLEROPROTEINS
SKELETAL DISEASES
SOMATIC CELLS
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques