Assignment of selected hyperfine proton NMR resonances in the met forms of Glycera dibranchiata monomer hemoglobins and comparisons with sperm whale metmyoglobin
This work indicates a high degree of purity for our preparations of all three of the primary Glycera dibranchiata monomer hemoglobins and details assignments of the heme methyl and vinyl protons in the hyperfine shift region of the ferric (aquo.) protein forms. The assignments were carried out by reconstituting the apoproteins of each component with selectively deuteriated hemes. The results indicate that even though the individual component preparations consist of essentially a single protein, the proton NMR spectra indicate spectroscopic heterogeneity. Evidence is presented for identification and classification of major and minor protein forms that are present in solutions of each component. Finally, in contrast to previous results, a detailed analysis of the proton hyperfine shift patterns of the major and minor forms of each component, in comparison to the major and minor forms of metmyoglobin, leads to the conclusions that the corresponding forms of the proteins from each species have strikingly similar heme-globin contacts and display nearly identical heme electronic structures and coordination numbers.
- Research Organization:
- Univ. of New Mexico, Albuquerque (USA)
- OSTI ID:
- 6999617
- Journal Information:
- Biochemistry; (United States), Vol. 27:8
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HEMOGLOBIN
NUCLEAR MAGNETIC RESONANCE
ANNELIDS
CETACEANS
DEUTERIUM COMPOUNDS
ERYTHROCYTES
HYPERFINE STRUCTURE
METHEMOGLOBIN
PROTONS
ANIMALS
AQUATIC ORGANISMS
BARYONS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
ELEMENTARY PARTICLES
FERMIONS
GLOBIN
HADRONS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
INVERTEBRATES
MAGNETIC RESONANCE
MAMMALS
MATERIALS
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PROTEINS
RESONANCE
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics