The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells

Mohr, F C; Fewtrell, C

Title: The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells

Full Record
Other Related Research

Abstract

The depletion of intracellular ATP by mitochondrial inhibitors in a glucose-free saline solution inhibited antigen-stimulated 45Ca uptake, the rise in cytoplasmic calcium, measured by fura-2, and secretion in rat basophilic leukemia cells. Lowering the intracellular ATP concentration also released calcium from an intracellular store and made further 45Ca efflux from the cells unresponsive to subsequent antigen stimulation. Antigen-stimulated 45Ca efflux could be restored by the addition of glucose. The ATP-sensitive calcium store appeared to be the same store that releases calcium in response to antigen. In contrast, intracellular ATP was not lowered, and antigen-stimulated secretion was unaffected by mitochondrial inhibitors, provided that glucose was present in the bathing solution. Similarly, antigen-stimulated 45Ca uptake, 45Ca efflux, and the rise in free ionized calcium were unaffected by individual mitochondrial inhibitors in the presence of glucose. However, when the respiratory chain inhibitor antimycin A was used in combination with the ATP synthetase inhibitor oligomycin in the presence of glucose, antigen-stimulated 45Ca uptake was inhibited, whereas the rise in free ionized calcium and secretion were unaffected. Also, antigen-induced depolarization (an indirect measurement of Ca2+ influx across the plasma membrane) was not affected. The inhibition of antigen-stimulated 45Ca uptake could, however, be overcome if amore »« less

Authors:

Mohr, F C; Fewtrell, C ^[1]

Cornell Univ., Ithaca (USA)

Publication Date:: Thu Feb 01 00:00:00 EST 1990

OSTI Identifier:: 6995015

Resource Type:: Journal Article

Journal Name:: American Journal of Physiology; (USA)

Additional Journal Information:: Journal Volume: 258; Journal ID: ISSN 0002-9513

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; ANTIGENS; BIOLOGICAL FUNCTIONS; CALCIUM; MEMBRANE TRANSPORT; ANTIMYCIN; ATP; CALCIUM 45; ENZYME INHIBITORS; FLUORESCENCE; GLUCOSE; HOMEOSTASIS; LEUKEMIA; MAST CELLS; MITOCHONDRIA; RATS; SURVIVAL TIME; TRACER TECHNIQUES; TUMOR CELLS; ALDEHYDES; ALKALINE EARTH ISOTOPES; ALKALINE EARTH METALS; ANIMAL CELLS; ANIMALS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CALCIUM ISOTOPES; CARBOHYDRATES; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DISEASES; DRUGS; ELEMENTS; EVEN-ODD NUCLEI; FUNCTIONS; HEMIC DISEASES; HEXOSES; IMMUNE SYSTEM DISEASES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LUMINESCENCE; MAMMALS; METALS; MONOSACCHARIDES; NEOPLASMS; NUCLEI; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANOIDS; RADIOISOTOPES; RODENTS; SACCHARIDES; SOMATIC CELLS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats


                    Mohr, F C, and Fewtrell, C. The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells.  United States: N. p., 1990. 
        Web.

Copy to clipboard


                    Mohr, F C, & Fewtrell, C. The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells.  United States.

Copy to clipboard


                    Mohr, F C, and Fewtrell, C. 1990.  
        "The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells".  United States.

Copy to clipboard


                    
@article{osti_6995015,

  title        = {The effect of mitochondrial inhibitors on calcium homeostasis in tumor mast cells},

  author       = {Mohr, F C and Fewtrell, C},

  abstractNote = {The depletion of intracellular ATP by mitochondrial inhibitors in a glucose-free saline solution inhibited antigen-stimulated 45Ca uptake, the rise in cytoplasmic calcium, measured by fura-2, and secretion in rat basophilic leukemia cells. Lowering the intracellular ATP concentration also released calcium from an intracellular store and made further 45Ca efflux from the cells unresponsive to subsequent antigen stimulation. Antigen-stimulated 45Ca efflux could be restored by the addition of glucose. The ATP-sensitive calcium store appeared to be the same store that releases calcium in response to antigen. In contrast, intracellular ATP was not lowered, and antigen-stimulated secretion was unaffected by mitochondrial inhibitors, provided that glucose was present in the bathing solution. Similarly, antigen-stimulated 45Ca uptake, 45Ca efflux, and the rise in free ionized calcium were unaffected by individual mitochondrial inhibitors in the presence of glucose. However, when the respiratory chain inhibitor antimycin A was used in combination with the ATP synthetase inhibitor oligomycin in the presence of glucose, antigen-stimulated 45Ca uptake was inhibited, whereas the rise in free ionized calcium and secretion were unaffected. Also, antigen-induced depolarization (an indirect measurement of Ca2+ influx across the plasma membrane) was not affected. The inhibition of antigen-stimulated 45Ca uptake could, however, be overcome if a high concentration of the Ca2+ buffer quin2 was present in the cells to buffer the incoming 45Ca. These results suggest that in fully functional rat basophilic leukemia cells the majority of the calcium entering in response to antigen stimulation is initially buffered by a calcium store sensitive to antimycin A and oligomycin, presumably the mitochondria.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/6995015},
  journal      = {American Journal of Physiology; (USA)},

  issn         = {0002-9513},

  number       = ,

  volume       = 258,

  place        = {United States},

  year         = {Thu Feb 01 00:00:00 EST 1990},

  month        = {Thu Feb 01 00:00:00 EST 1990}

}

Copy to clipboard

Journal Article:

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

IgE receptor-activated calcium permeability pathway in rat basophilic leukemia cells: measurement of the unidirectional influx of calcium using quin2-buffered cells

Journal Article Fewtrell, C; Sherman, E - Biochemistry; (United States)

The intracellular calcium indicator and buffer quin2 has been used to generate a large calcium buffering capacity in the cytoplasm of rat basophilic leukemia cells. Above 3 mM intracellular quin2, there is no further increase in the initial rate of antigen-induced /sup 45/Ca uptake, suggesting that /sup 45/Ca buffering by quin2 is now sufficient to prevent the immediate efflux of /sup 45/Ca from the cells. Thus, the initial rate of /sup 45/Ca uptake should reflect the true unidirectional influx of calcium that occurs when innumoglobulin E (IgE) receptors are aggregated by antigen. The antigen-induced calcium permeability pathway appears to bemore »« less
https://doi.org/10.1021/bi00396a021
Regulation of intracellular calcium in resting and stimulated rat basophilic leukemia cells

Thesis/Dissertation Mohr, F

Intracellular calcium regulation was studied in a cell line of mast cells, the rat basophilic leukemia (RBL) cells with the purpose of determining (1) The properties of the plasma membrane calcium permeability pathway and (2) The role of intracellular calcium stores. The first set of experiments showed that depolarization did not induce calcium entry or secretion in resting cells and did inhibit antigen-stimulated calcium uptake and secretion. In the second set of experiments the ionic basis of antigen-induced depolarization was studied using the fluorescent potential-sensitive probe bis-oxonol. The properties of the calcium entry pathway were more consistent with a calciummore »« less
A new role for follicle-stimulating hormone in the regulation of calcium flux in Sertoli cells: Inhibition of Na+/Ca++ exchange

Journal Article Grasso, P; Joseph, M; Reichert, Jr, L - Endocrinology; (USA)

Elucidation of mechanisms regulating intracellular calcium levels in steroidogenic tissues is important for understanding control of cellular function. We have previously described FSH receptor-mediated flux of 45Ca++ into cultured rat Sertoli cells and receptor-enriched proteoliposomes via voltage-sensitive and voltage-independent calcium channels. In the present study, we report heretofore unrecognized inhibitory effects of FSH on Na+/Ca++ exchange in these two systems. An outwardly directed Na+ gradient, developed by preincubating Sertoli cell monolayers in buffer made hypertonic with NaCl, resulted in uptake of 45Ca++ that was unaffected by calcium channel blocking agents, ruthenium red or methoxyverapamil, but was enhanced by ouabain, amore »« less
https://doi.org/10.1210/endo-128-1-158
IgE receptor-activated calcium permeability pathway in rat basophilic leukemia cells

Conference Fewtrell, C; Sherman, E; Mohr, F - Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

When antigen-stimulated /sup 45/Ca uptake is measured in RBL cells loaded with > 3 mM quin2, re-extrusion of /sup 45/Ca is minimized and the initial rate of /sup 45/Ca uptake reflects the true unidirectional influx of Ca. This influx correlates more closely with secretion than with the number of IgE receptors aggregated by antigen. The antigen-induced permeability pathway is saturable, having a Km of about 0.7 mM and a Vmax of 0.9 nmol Ca/10/sup 6/ cells/min and it persists for at least an hour provided that receptor aggregation is maintained. The negatively charged fluorescent probe bis-oxonol is insensitive to changesmore »« less
Evidence for a calcium-mobilizing receptor triggered by cadmium, or decreasing external pH or sodium in coronary endothelial cells

Conference Dwyer, S; Zhuang, Yingxin; Smith, L; ... - FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

Cadmium (1 {mu}M) or decreasing external pH (pH{sub o}) or Na concentration (Na{sub o}) transiently increased cytoplasmic free Ca (Ca{sub i}) by {approximately}5-fold as measured with fura-2 in endothelial cells grown on cover glasses. Cd or pH{sub o}=6 evoked similar peak Ca{sub i} increases in the presence or absence of external Ca indicating that they trigger the release of stored Ca. Cd or 4 mM Na{sub o}, but not pH{sub o} 6, caused sustained Ca{sub i} increases. The sustained phase of the Ca{sub i} response to Cd required external Ca. Decreasing intracellular pH with propionate had no effect on Ca{submore »« less

Similar Records