Opiate-prostaglandin interactions in the regulation of insulin secretion from rat islets of Langerhans in vitro

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(88)90126-9· OSTI ID:6994955

Green, I C; Tadayyon, M

The inadequate insulin secretory response to glucose stimulation in non-insulin dependent diabetes has been attributed to many factors including high PGE/sub 2/ levels blunting the secretory response, and to the existence of inhibitory opiate activity in vivo. The purpose of the present work was to see if there was a connection between these two independent theories. Radioimmunoassayable PGE/sub 2/ in islets of Langerhans was found to be proportional to islet number and protein content and was typically 4 to 5pg/..mu..g islet protein. Indomethacin sodium salicylate and chlorpropamide all lowered islet PGE/sub 2/ levels and stimulated insulin release in vitro. Dynorphin stimulated insulin release at a concentration of 6 x 10/sup -9/M, while lowering islet PGE/sub 2/. Conversely, at a higher concentration, dynorphin had no stimulatory effect on insulin secretion and did not lower PGE/sub 2/ levels in islets or in the incubation media. The stimulatory effects of dynorphin and sodium salicylate on insulin secretion were blocked by exogenous PGE/sub 2/. PGE/sub 2/ at a lower concentration did not exert any inhibitory effect on dynorphin- or sodium salicylate-induced insulin release. This concentration of exogenous PGE/sub 2/ stimulated insulin release in the presence of 6mM glucose.

• Research Organization: Univ. of Sussex, Brighton (England)
• OSTI ID: 6994955
• Journal Information: Life Sci.; (United States), Vol. 42:21
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DIABETES MELLITUS
PATHOGENESIS
INSULIN
SECRETION
PROSTAGLANDINS
BIOCHEMICAL REACTION KINETICS
IN VITRO
PANCREAS
RADIOIMMUNOASSAY
RATS
ANIMALS
BODY
DIGESTIVE SYSTEM
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
METABOLIC DISEASES
ORGANS
PEPTIDE HORMONES
RADIOASSAY
RADIOIMMUNOLOGY
REACTION KINETICS
RODENTS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550901 - Pathology- Tracer Techniques