Effects of beta-carotene and vitamin A on bovine luteal function
Initially, the direct effects of B-carotene and vitamin A on progesterone (P4) production were studied by exposing dispersed luteal cells to these compounds in vitro. There were no positive relationships between P4 and B-carotene or vitamin A. However, a negative, and perhaps toxic, effect of a large dose of B-carotene on P4 reproduction was noted. A positive relationship between plasma B-carotene and percent change of P4 in the medium of dispersed luteal cells was demonstrated when these plasma metabolites were measured in slaughterhouse cows from which CL were obtained for incubation. This relationship was only present during the winter when plasma levels of B-carotene and vitamin A were considerably lower. Preliminary investigations into the mechanism of action of B-carotene and/or vitamin A were initiated. Luteal tissue ribonucleic acid (RNA), deoxyribonucleic acid (DNA), the RNA to DNA ratio and total protein concentration were measured to study the influence of plasma levels of B-carotene and vitamin A on the protein synthetic capacity of luteal tissue. There were no relationships detected, however, RNA concentration and the RNA to DNA ratio of luteal tissue were greater during the summer. The percent binding of radiolabeled vitamin A was greater in the nuclear than in the cytoplasmic component of the luteal cell.
- Research Organization:
- Connecticut Univ., Storrs (USA)
- OSTI ID:
- 6994825
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CAROTENOIDS
BIOLOGICAL EFFECTS
OVARIES
BIOLOGICAL FUNCTIONS
PROGESTERONE
BIOSYNTHESIS
VITAMIN A
CATTLE
CELL NUCLEI
CYTOPLASM
DNA
RADIOISOTOPES
RNA
SEASONAL VARIATIONS
TRACER TECHNIQUES
ANIMALS
BODY
CELL CONSTITUENTS
DOMESTIC ANIMALS
FEMALE GENITALS
FUNCTIONS
GONADS
HORMONES
HYDROCARBONS
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PIGMENTS
PREGNANES
RUMINANTS
STEROID HORMONES
STEROIDS
SYNTHESIS
TERPENES
VARIATIONS
VERTEBRATES
VITAMINS
550201* - Biochemistry- Tracer Techniques