Sequence-specific assignments in the /sup 1/H NMR spectrum of the human inflammatory protein C5a

Journal Article · Tue May 17 00:00:00 EDT 1988 · Biochemistry; (United States)

OSTI ID:6994209

Zuiderweg, E R.P.; Mollison, K W; Henkin, J; Carter, G W

Full sequence-specific assignments for the /sup 1/H NMR lines of the backbone protons of the human complement factor C5a are described and documented. The results were obtained by largely following the methodology developed by Wuethrich et al. Assignments for the majority of the amino acid side chain protons were obtained by using a comparison of double- and triple-quantum-filtered two-dimensional correlated experiments together with the analysis of relayed coherence transfer spectra. The assignments provide the basis for the determination of the thus far unknown three-dimensional structure of C5a from nuclear Overhauser enhancement distance constraints.

• Research Organization: Abbott Labs., Abbott Parks, IL (USA)
• OSTI ID: 6994209
• Journal Information: Biochemistry; (United States), Vol. 27:10
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
INFLAMMATION
BIOCHEMISTRY
PROTEINS
NUCLEAR MAGNETIC RESONANCE
MAN
OVERHAUSER EFFECT
PROTONS
ANIMALS
BARYONS
CHEMISTRY
ELEMENTARY PARTICLES
FERMIONS
HADRONS
MAGNETIC RESONANCE
MAMMALS
NUCLEONS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PRIMATES
RESONANCE
SYMPTOMS
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics