Nucleotide sequence of a complementary DNA encoding pea cytosolic copper/zinc superoxide dismutase. [Pisum sativum L]

Journal Article · Thu Aug 01 00:00:00 EDT 1991 · Plant Physiology; (United States)

DOI:https://doi.org/10.1104/pp.96.4.1391· OSTI ID:6994045

White, D A; Zilinskas, B A ^[1]

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1. Rutgers, The State Univ. of New Jersey, New Brunswick (United States)

The authors now report the nucleotide sequence of the cytosolic Cu/Zn SOD cloned from a {lambda}gt11 cDNA library constructed from mRNA extracted from leaves of 7- to 10-d pea seedlings (Pisum sativum L.). The clone was isolated using a 22-base synthetic oligonucleotide complementary to the amino acid sequence CGIIGLQG. This sequence, found at the protein's carboxy terminus, is highly conserved among plant cytosolic Cu/Zn SODs but not chloroplastic Cu/Zn SODs. The 738-base pair sequence contains an open reading frame specifying 152 codons and a predicted M{sub r} of 18,024 D. The deduced amino acid sequence is highly homologous (79-82% identity) with the sequences of other known plant cytosolic Cu/Zn SODs but less highly conserved (63-65%) when compared with several chloroplastic Cu/Zn SODs including pea (10).

• OSTI ID: 6994045
• Journal Information: Plant Physiology; (United States), Vol. 96:4; ISSN 0032-0889
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PISUM
MOLECULAR BIOLOGY
SUPEROXIDE DISMUTASE
DNA SEQUENCING
AMINO ACID SEQUENCE
COPPER
DNA-CLONING
LEAVES
METALLOPROTEINS
SEEDLINGS
ZINC
CLONING
DNA HYBRIDIZATION
ELEMENTS
ENZYMES
HYBRIDIZATION
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
METALS
MOLECULAR STRUCTURE
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENTS
550200* - Biochemistry