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Title: Effects of X-ray irradiation on natural killer (NK) cell system. II. Increased sensitivity to natural killer cytotoxic factor (NKCF)

Irradiation with low-doses of X-rays of tumor cells elevated their susceptibility to lysis by natural killer (NK) cells in an accompanying paper. Cytotoxicity assays conducted at the single cell level revealed that X-ray irradiation of K562 cells did not affect the number of effector-target conjugates but increased the frequency of dead conjugated target cells. During interaction with K562 cells large granular lymphocytes released a soluble cytotoxic factor (NKCF) that killed the target cells. X-ray irradiation did not affect the NKCF stimulatory ability of K562 cells, while it elevated their sensitivity to the lytic effect of NKCF. In contrast to X-rays, exposure to ultraviolet (UV) radiation of K562 cells did not elevate their NK sensitivity but rather reduced it. Treatment with mitomycin C produced no effect on NK sensitivity. These results indicate that X-ray irradiation elevates the target sensitivity to NKCF, which may be involved in the increased NK sensitivity, and that the X-ray effect may be different from that of UV radiation or DNA synthesis inhibition.
Authors:
; ; ;  [1]
  1. (Kyoto Univ. (Japan))
Publication Date:
OSTI Identifier:
6992753
Resource Type:
Journal Article
Resource Relation:
Journal Name: Immunopharmacology and Immunotoxicology; (USA); Journal Volume: 11:2-3
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; CELL PROLIFERATION; TUMOR CELLS; BIOLOGICAL RADIATION EFFECTS; COMPARATIVE EVALUATIONS; LYSIS; MITOMYCIN; ULTRAVIOLET RADIATION; X RADIATION; ANIMAL CELLS; ANTI-INFECTIVE AGENTS; ANTIBIOTICS; ANTIMITOTIC DRUGS; ANTINEOPLASTIC DRUGS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CONNECTIVE TISSUE CELLS; DRUGS; ELECTROMAGNETIC RADIATION; IONIZING RADIATIONS; LEUKOCYTES; MATERIALS; RADIATION EFFECTS; RADIATIONS; SOMATIC CELLS 560120* -- Radiation Effects on Biochemicals, Cells, & Tissue Culture