Quantitative role of the splanchnic bed in whole body leucine metabolism

Conference · Wed Mar 05 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:6991144

Yu, Y M; Wagner, D A; Tredget, E; Walesreweski, J; Burke, J F; Young, V R

The role of the splanchnic bed in the economy of whole body leucine (leu) metabolism was assessed in 5 chronically catheterized conscious fasting mongrel dogs. Using primed continuous intravenous infusions of L-(/sup 15/N, 1-/sup 13/C)-leu and L-1-/sup 14/C-leu the metabolic fate of leu carbon (C) and nitrogen (N) in the splanchnic region was compared with that in the body as a whole, by measurement of isotope and substrate balance across gut and liver. Sampling was from the portal and hepatic veins and arch of aorta. Blood flow estimation was made by dye dilution. Whole body leu N and C fluxes and oxidation were (Mean +/- SEM); 453 +/ 47, 197 +/- 37 and 41 +/- 5 ..mu..mol kg-1.h-1, respectively. The splanchnic bed accounted for (% of whole body) 36 +/- 13 of leu disappearance into proteins (liver 14%; gut 22%); 24 +/- 7 of leu appearance via protein breakdown (liver 8%; gut 16%) 12 +/- 2% of leu transamination to ..cap alpha..-ketoisocaproate (KIC) (liver 7%; gut 5%); 12 +/- 3 of KIC reamination to leu (liver 7%; gut 5%) and 11 +/- 3 of leu oxidation (liver 2%; gut 9%). Hence, in the fasting state the splanchnic region accounts for a small proportion of whole body leu-KIC interconversion and oxidation, but a more significant proportion of whole body of leu for protein synthesis.

• Research Organization: Massachusetts General Hospital, Boston
• OSTI ID: 6991144
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LEUCINE
METABOLISM
BIOLOGICAL FUNCTIONS
BLOOD FLOW
CARBON 13
CARBON 14
DOGS
LIVER
NITROGEN 15
TRACER TECHNIQUES
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBON ISOTOPES
CARBOXYLIC ACIDS
DIGESTIVE SYSTEM
EVEN-EVEN NUCLEI
EVEN-ODD NUCLEI
FUNCTIONS
GLANDS
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MAMMALS
NITROGEN ISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIOISOTOPES
STABLE ISOTOPES
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550501* - Metabolism- Tracer Techniques