myo-Inositol synthesis from (1-/sup 3/H)glucose in Phaseolus vulgaris L. during early stages of germination

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · Plant Physiol.; (United States)

OSTI ID:6990272

Sasaki, K; Taylor, I E.P.

Radiolabeled D-(1-/sup 3/H)glucose was fed by imbibition under sterile conditions to bean (Phaseolus vulgaris L.) seeds. After 72 and 96 hours of feeding, the /sup 3/H was located in uronic acid and pentose residues as well as hexose residues of cell wall polysaccharides in growing hypocotyl and root. Free myo-inositol present in cotyledons, hypocotyl, and root also contained /sup 3/H, showing that de novo synthesis of myo-inositol from (1-/sup 3/H)glucose did occur during the first 72 hours of germination. More than 90% of the labeled, free myo-inositol was present in the cotyledons. The /sup 3/H percentage in trifluoroacetic acid-soluble arabinaose residues of cell wall polysaccharides from 72-hour-old bean hypocotyls was only half of their mole percentage. On the other hand, /sup 3/H percentages in hexose residues were higher than their mole percentages. The results suggest that myo-inositol is synthesized from reserve sugars during the very early stages of germination, and that the newly synthesized myo-inositol, as well as that stored in cotyledons, can be used for the construction of new hypocotyl and root cell wall polysaccharides after conversion into uronic acids and pentoses via the myo-inositol oxidation pathway.

• Research Organization: Univ. of British Columbia, Vancouver
• OSTI ID: 6990272
• Journal Information: Plant Physiol.; (United States), Vol. 81:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLUCOSE
BIOCHEMICAL REACTION KINETICS
INOSITOL
BIOSYNTHESIS
PHASEOLUS
PLANT GROWTH
GERMINATION
SEEDS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALDEHYDES
BACTERIA
CARBOHYDRATES
GROWTH
HEXOSES
INOSITOLS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
LEGUMINOSAE
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
RHIZOBIUM
SACCHARIDES
SYNTHESIS
551001* - Physiological Systems- Tracer Techniques