Distinct accessory cell requirements define two types of rat T cell hybridomas specific for unique determinants in the encephalitogenic 68-86 region of myelin basic protein

Journal Article · Mon Jan 15 00:00:00 EST 1990 · Journal of Immunology; (USA)

OSTI ID:6983462

Mannie, M D; Paterson, P Y; Thomas, D W; Nairn, R ^[1]

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1. Univ. of Michigan Medical School, Ann Arbor (USA)

Six clonotypically unique T cell hybridomas from Lewis rats were used to study accessory cell activities required for class II MHC restricted T cell responses to the 68-86 encephalitogenic sequence of myelin basic protein (MBP). T cell hybrids which were cultured with GP68-86 68-86 sequence of guinea pig MBP (GPMBP) and naive splenocytes (SPL) were induced to produce IL-2 as measured by the CTLL indicator cell line. The hybrids were categorized into two subsets (designated THYB-1 and THYB-2), because two distinct subset-specific pathways of communication between accessory cells and T cells were involved in GPMBP-induced IL-2 production. These pathways were distinguished by the following six observations. First, when the duration of a pulse of SPL with GPMBP was lengthened from 1 to 4 h, these SPL lost their ability to induce IL-2 production by THYB-2 hybrids yet nevertheless retained full stimulatory activity for THYB-1 hybrids. Second, paraformaldehyde fixation of GPMBP-pulsed SPL abrogated an activity necessary for Ag-induced IL-2 production by THYB-2 hybrids. These fixed SPL were nevertheless able to stimulate THYB-1 hybrids, albeit to a lesser extent than viable unfixed SPL. Third, the addition of either cycloheximide, cytochalasin B, or 2-deoxyglucose to an Ag pulse of SPL with GPMBP dramatically inhibited the subsequent responses of THYB-2 hybrids yet had little or no effect upon the reactivity of THYB-1 hybrids. Fourth, thymocytes lacked necessary activities for GPMBP evoked IL-2 production by THYB-2 hybrids yet strongly promoted THYB-1 hybrid responses. Fifth, exposure of SPL to as little as 500 rad of gamma-irradiation markedly attenuated THYB-2 hybrid response to GPMBP but did not affect THYB-1 responses. Sixth, anti-GPMBP responses by THYB-2 hybrids were observed only in the presence of both radioresistant adherent SPL and a distinct population of radiosensitive nonadherent SPL.

• OSTI ID: 6983462
• Journal Information: Journal of Immunology; (USA), Vol. 144:2; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYBRIDOMAS
SPECIFICITY
LYMPHOKINES
BIOSYNTHESIS
SPLEEN CELLS
BIOLOGICAL RADIATION EFFECTS
ANTIGENS
BIOLOGICAL PATHWAYS
CLONE CELLS
ENCEPHALITIS
GAMMA RADIATION
LYMPHOCYTES
PEPTIDES
RATS
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CULTURES
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
GROWTH FACTORS
INFECTIOUS DISEASES
IONIZING RADIATIONS
LEUKOCYTES
MAMMALS
MATERIALS
MITOGENS
NERVOUS SYSTEM DISEASES
ORGANIC COMPOUNDS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
VIRAL DISEASES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture