Studies on the solution conformation of human thioredoxin using heteronuclear sup 15 N- sup 1 H nuclear magnetic resonance spectroscopy

Journal Article · Tue Feb 13 00:00:00 EST 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00458a030· OSTI ID:6976165

Forman-Kay, J D ^[1]; Gronenborn, A M; Kay, L E; Clore, G M ^[2]; Wingfield, P T ^[3]

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1. National Institutes of Health, Bethesda, MD (USA) Yale Univ., New Haven, CT (USA)
2. National Institutes of Health, Bethesda, MD (USA)
3. Glaxo Institute for Molecular Biology, Geneva (Switzerland)

The solution conformation of uniformly labeled {sup 15}N human thioredoxin has been studied by two-dimensional heteronuclear {sup 15}N-{sup 1}H nuclear magnetic resonance spectroscopy. Assignments of the {sup 15}N resonances of the protein are obtained in a sequential manner using heteronuclear multiple quantum coherence (HMQC), relayed HMQC-correlated (COSY), and relayed HMQC-nuclear Overhauser (NOESY) spectroscopy. Values of the {sup 3}J{sub HN{alpha}} splittings for 87 of the 105 residues of thioredoxin are extracted from a variant of the HMQC-COSY experiment, known as HMQC-J, and analyzed to give accurate {sup 3}J{sub HN{alpha}} coupling constants. In addition, long-range C{sub {alpha}}H(i)-{sup 15}N(i+1) scalar connectivities are identified by heteronuclear multiple bond correlation (HMBC) spectroscopy. The presence of these three-bond scalar connectivities in predominantly {alpha}-helical regions correlates well with the secondary structure determined previously from a qualitative analysis of homonuclear nuclear Overhauser data suggesting that this technique may provide additional information for secondary structure determination a priori. The accuracy with which {sup 3}J{sub HN{alpha}} coupling constants can be obtained from the HMQC-J experiment permits a more precise delineation of the beginnings and ends of secondary structural elements of human thioredoxin and of irregularities in these elements.

• OSTI ID: 6976165
• Journal Information: Biochemistry; (USA), Vol. 29:6; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
PROTEINS
NUCLEAR MAGNETIC RESONANCE
AQUEOUS SOLUTIONS
CHEMICAL SHIFT
CONFORMATIONAL CHANGES
ESCHERICHIA COLI
MAN
MOLECULAR STRUCTURE
NITROGEN 15
OVERHAUSER EFFECT
PROTONS
THIOLS
ANIMALS
BACTERIA
BARYONS
DISPERSIONS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MICROORGANISMS
MIXTURES
NITROGEN ISOTOPES
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
RESONANCE
SOLUTIONS
STABLE ISOTOPES
VERTEBRATES
550601* - Medicine- Unsealed Radionuclides in Diagnostics