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Title: Distribution of IGF receptors in the plasma membrane of proximal tubular cells

Abstract

To characterize the distribution of receptors for insulin-like growth factors I and II (IGF I and II) in the plasma membrane of the renal proximal tubular cell, the authors measured binding of {sup 125}I-labeled IGF I and {sup 125}I-labeled IGF II to proximal tubular basolateral and brush-border membranes and characterized IGF I-stimulated phosphorylation of detergent-solubilized membranes. {sup 125}I-IGF bound primarily to a 135,000 relative molecular weight (M{sub r}) protein and IGF II to a 260,000 M{sub r} protein in isolated membranes. Binding of {sup 125}I-IGF I was severalfold greater in basolateral than in brush-border membranes. IGF I-stimulated phosphorylation of the 92,000 M{sub r} {beta}-subunit of its receptors could be demonstrated only in basolateral membranes. These findings are consistent with an asymmetrical distribution of receptors for IGF I in the plasma membrane of the renal proximal tubular cell, localization being primary on the basolateral side. In contrast, binding of {sup 125}I-IGF II to isolated basolateral and brush-border membranes was equivalent, suggesting that receptors for this peptide are distributed more symmetrically in the plasma membrane. The findings suggest that the action of IGF I in proximal tubule are mediated via interaction of circulating peptide with specific receptors in the basolateral membrane. However,more » the findings established the potential for actions of IGF II to be exerted in proximal tubule via interaction with both basolateral and/or brush-border membrane receptors.« less

Authors:
;  [1]
  1. Washington Univ. School of Medicine, St. Louis, MO (USA)
Publication Date:
OSTI Identifier:
6974663
Resource Type:
Journal Article
Journal Name:
American Journal of Physiology; (USA)
Additional Journal Information:
Journal Volume: 253:5; Journal ID: ISSN 0002-9513
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL MEMBRANES; AUTORADIOGRAPHY; GROWTH FACTORS; RADIORECEPTOR ASSAY; RECEPTORS; TISSUE DISTRIBUTION; TUBULES; PHYSIOLOGY; BIOCHEMICAL REACTION KINETICS; DOSE-RESPONSE RELATIONSHIPS; ELECTROPHORESIS; INSULIN; IODINE 125; PHOSPHOTRANSFERASES; BETA DECAY RADIOISOTOPES; BODY; CELL CONSTITUENTS; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; KIDNEYS; KINETICS; MEMBRANE PROTEINS; MEMBRANES; MITOGENS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PEPTIDE HORMONES; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; TRACER TECHNIQUES; TRANSFERASES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Hammerman, M R, and Rogers, S. Distribution of IGF receptors in the plasma membrane of proximal tubular cells. United States: N. p., 1987. Web.
Hammerman, M R, & Rogers, S. Distribution of IGF receptors in the plasma membrane of proximal tubular cells. United States.
Hammerman, M R, and Rogers, S. 1987. "Distribution of IGF receptors in the plasma membrane of proximal tubular cells". United States.
@article{osti_6974663,
title = {Distribution of IGF receptors in the plasma membrane of proximal tubular cells},
author = {Hammerman, M R and Rogers, S},
abstractNote = {To characterize the distribution of receptors for insulin-like growth factors I and II (IGF I and II) in the plasma membrane of the renal proximal tubular cell, the authors measured binding of {sup 125}I-labeled IGF I and {sup 125}I-labeled IGF II to proximal tubular basolateral and brush-border membranes and characterized IGF I-stimulated phosphorylation of detergent-solubilized membranes. {sup 125}I-IGF bound primarily to a 135,000 relative molecular weight (M{sub r}) protein and IGF II to a 260,000 M{sub r} protein in isolated membranes. Binding of {sup 125}I-IGF I was severalfold greater in basolateral than in brush-border membranes. IGF I-stimulated phosphorylation of the 92,000 M{sub r} {beta}-subunit of its receptors could be demonstrated only in basolateral membranes. These findings are consistent with an asymmetrical distribution of receptors for IGF I in the plasma membrane of the renal proximal tubular cell, localization being primary on the basolateral side. In contrast, binding of {sup 125}I-IGF II to isolated basolateral and brush-border membranes was equivalent, suggesting that receptors for this peptide are distributed more symmetrically in the plasma membrane. The findings suggest that the action of IGF I in proximal tubule are mediated via interaction of circulating peptide with specific receptors in the basolateral membrane. However, the findings established the potential for actions of IGF II to be exerted in proximal tubule via interaction with both basolateral and/or brush-border membrane receptors.},
doi = {},
url = {https://www.osti.gov/biblio/6974663}, journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 253:5,
place = {United States},
year = {Sun Nov 01 00:00:00 EST 1987},
month = {Sun Nov 01 00:00:00 EST 1987}
}