Na sup + -H sup + exchanger in proximal cells isolated from rabbit kidney. I. Functional characteristics
Abstract
The purpose of this study was to investigate the characteristics of the Na{sup +}-H{sup +} exchange in isolated proximal cells from rabbit kidney cortex. The cells were prepared by mechanical dissociation and sequential passages through nylon meshes. The intracellular pH (pH{sub i}) was measured in a bicarbonate-free medium using the fluorescent dye 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF). Resting pH{sub i} was 7.13 {+-} 0.04. Cells were acid loaded with nigericin in choline solution and H{sup +} efflux, induced by extracellular Na{sup +} (Na{sub e}), was calculated using a buffering power of 23.6 {+-} 0.6 mmol {center dot} l{sup {minus}1} {center dot} pH unit{sup {minus}1} estimated by NH{sub 4}Cl exposure. The intracellular H{sup +} concentration dependence did not follow simple Michaelis-Menten kinetics. Of the different cations tested on pH{sub i} recovery, such as Li{sup +}, choline{sup +}, K{sup +}, and tetramethylammonium, only Li{sup +} induced an alkalinization of acidified cells similar to that of Na{sup +}. {sup 22}Na influx measurements indicated that cellular depletion of Na{sup +} stimulated Na{sup +}-H{sup +} exchange. The results permit the conclusion that the isolation procedures did not impair the main features of the Na{sup +}-H{sup +} antiporter, at least as compared with those previously described in renal brush-bordermore »
- Authors:
-
- Institut National de la Sante et de la Recherche Medicale (France)
- Publication Date:
- OSTI Identifier:
- 6974620
- Resource Type:
- Journal Article
- Journal Name:
- American Journal of Physiology; (USA)
- Additional Journal Information:
- Journal Volume: 253:5; Journal ID: ISSN 0002-9513
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; FLUORESCEIN; EMISSION SPECTRA; MEMBRANE PROTEINS; BIOCHEMISTRY; MEMBRANE TRANSPORT; BIOCHEMICAL REACTION KINETICS; SODIUM 22; UPTAKE; ACID CARBONATES; CATIONS; KIDNEYS; PH VALUE; PROTONS; RABBITS; SODIUM CHLORIDES; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; ANIMALS; AROMATICS; BARYONS; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CARBOXYLIC ACIDS; CHARGED PARTICLES; CHEMISTRY; CHLORIDES; CHLORINE COMPOUNDS; DYES; ELEMENTARY PARTICLES; FERMIONS; HADRONS; HALIDES; HALOGEN COMPOUNDS; HYDROXY ACIDS; HYDROXY COMPOUNDS; IONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPES; KINETICS; LIGHT NUCLEI; MAMMALS; NUCLEI; NUCLEONS; ODD-ODD NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PHENOLS; POLYPHENOLS; PROTEINS; RADIOISOTOPES; REACTION KINETICS; SODIUM COMPOUNDS; SODIUM ISOTOPES; SPECTRA; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 551001* - Physiological Systems- Tracer Techniques
Citation Formats
Bidet, M, Tauc, M, Merot, J, Vandewalle, A, and Poujeol, P. Na sup + -H sup + exchanger in proximal cells isolated from rabbit kidney. I. Functional characteristics. United States: N. p., 1987.
Web.
Bidet, M, Tauc, M, Merot, J, Vandewalle, A, & Poujeol, P. Na sup + -H sup + exchanger in proximal cells isolated from rabbit kidney. I. Functional characteristics. United States.
Bidet, M, Tauc, M, Merot, J, Vandewalle, A, and Poujeol, P. 1987.
"Na sup + -H sup + exchanger in proximal cells isolated from rabbit kidney. I. Functional characteristics". United States.
@article{osti_6974620,
title = {Na sup + -H sup + exchanger in proximal cells isolated from rabbit kidney. I. Functional characteristics},
author = {Bidet, M and Tauc, M and Merot, J and Vandewalle, A and Poujeol, P},
abstractNote = {The purpose of this study was to investigate the characteristics of the Na{sup +}-H{sup +} exchange in isolated proximal cells from rabbit kidney cortex. The cells were prepared by mechanical dissociation and sequential passages through nylon meshes. The intracellular pH (pH{sub i}) was measured in a bicarbonate-free medium using the fluorescent dye 2,7-biscarboxyethyl-5(6)-carboxyfluorescein (BCECF). Resting pH{sub i} was 7.13 {+-} 0.04. Cells were acid loaded with nigericin in choline solution and H{sup +} efflux, induced by extracellular Na{sup +} (Na{sub e}), was calculated using a buffering power of 23.6 {+-} 0.6 mmol {center dot} l{sup {minus}1} {center dot} pH unit{sup {minus}1} estimated by NH{sub 4}Cl exposure. The intracellular H{sup +} concentration dependence did not follow simple Michaelis-Menten kinetics. Of the different cations tested on pH{sub i} recovery, such as Li{sup +}, choline{sup +}, K{sup +}, and tetramethylammonium, only Li{sup +} induced an alkalinization of acidified cells similar to that of Na{sup +}. {sup 22}Na influx measurements indicated that cellular depletion of Na{sup +} stimulated Na{sup +}-H{sup +} exchange. The results permit the conclusion that the isolation procedures did not impair the main features of the Na{sup +}-H{sup +} antiporter, at least as compared with those previously described in renal brush-border membrane vesicles or in other cellular systems. The integrity of the transporter in isolated proximal cells would permit the direct study of its hormonal and metabolic control.},
doi = {},
url = {https://www.osti.gov/biblio/6974620},
journal = {American Journal of Physiology; (USA)},
issn = {0002-9513},
number = ,
volume = 253:5,
place = {United States},
year = {Sun Nov 01 00:00:00 EST 1987},
month = {Sun Nov 01 00:00:00 EST 1987}
}