Optimizing carbon dioxide and light levels during in vitro culture of Theobroma cacao
- Purdue Univ., West Lafayette, IN (United States). Dept. of Horticulture
In vitro culture of axillary cotyledonary shoots of Theobroma cacao L. (cacao) under increasing CO[sub 2] concentration from ambient to 24,000 ppm (culture tube levels) significantly increased total shoot elongation, number of leaves, leaf area per explant, and shoot dry and fresh weight. Although light was necessary for the CO[sub 2] response, the effect of various photon fluxes was not significant for the measured growth parameters. Net photosynthesis estimated on the basis of CO[sub 2] depletion in culture tubes increased 3.5 times from 463 to 2639 ppm CO[sub 2], and increased 1.5 times from 2,639 to 14,849 ppm CO[sub 2], but declined from 14,849 to 24,015 ppm CO[sub 2]. Ethylene concentration in culture vessels increased under enriched CO[sub 2] conditions. Depletion of nutrients (fructose, K, Ca, Mg, and P) from the medium was increased under enriched CO[sub 2] conditions.
- OSTI ID:
- 6973667
- Journal Information:
- Journal of the American Society for Horticultural Science; (United States), Vol. 119:4; ISSN 0003-1062
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CACAO TREES
PLANT GROWTH
CARBON DIOXIDE
BIOLOGICAL EFFECTS
VISIBLE RADIATION
CARBON COMPOUNDS
CARBON OXIDES
CHALCOGENIDES
ELECTROMAGNETIC RADIATION
GROWTH
MAGNOLIOPHYTA
MAGNOLIOPSIDA
OXIDES
OXYGEN COMPOUNDS
PLANTS
RADIATIONS
TREES
560300* - Chemicals Metabolism & Toxicology