Observations on the contributions of environmental restraints and innate stem cell ability to hematopoietic regeneration
A competitive repopulation assay utilizing chromosome markers was used to assay the reconstituting potential of hematopoietic populations. The test populations consisted of tibial murine marrow locally irradiated with doses ranging from 1.5 Gy to 8.5 Gy and of marrow generated from either murine splenic or marrow stem cells. The purpose of this assay was to assess the innate proliferative potential and microenvironmental influences on the ability to repopulate. Regardless of origin, spleen repopulating ability consistently agreed with spleen colony-forming unit (CFU-s) content. Doses of radiation from 5 Gy to 8.5 Gy diminished, by a factor of 2, the ability to repopulate marrow despite maintenance of CFU-s levels. Marrow generated from splenic stem cells had one-fifth the repopulating ability of marrow derived from marrow stem cells, even though CFU-s levels were equivalent. The results imply that the splenic environment can only maintain stem cells at the level of the CFU-s, even if the stem cells were originally of higher quality, and that their original potential cannot be regained in a marrow environment. Nevertheless, the marrow can maintain more primitive stem cells, but this reserve is drained to support CFU-s levels.
- Research Organization:
- Hebrew Univ.-Hadassah Hospital Medical School, Jerusalem (Israel)
- OSTI ID:
- 6971604
- Journal Information:
- Int. J. Cell Cloning; (United States), Vol. 6:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Postirradiation glucan administration enhances the radioprotective effects of WR-2721
The effects of x-irradiation on hematopoietic stem cell compartments in the mouse
Related Subjects
SPLEEN CELLS
COLONY FORMATION
STEM CELLS
BIOLOGICAL RECOVERY
RADIOSENSITIVITY
BIOASSAY
BIOLOGICAL MARKERS
BONE MARROW
CELL PROLIFERATION
HEMATOPOIETIC SYSTEM
MICE
RADIATION DOSES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
DOSES
MAMMALS
ORGANS
RECOVERY
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture