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Title: Effect of immunologic reactions on rat intestinal epithelium. Correlation of increased permeability to chromium 51-labeled ethylenediaminetetraacetic acid and ovalbumin during acute inflammation and anaphylaxis

Abstract

In these studies we compared jejunal permeability to two probes--chromium 51-labeled ethylenediaminetetraacetic acid (51Cr-EDTA) (mol wt, 360) and ovalbumin (mol wt, 45,000)--under control conditions, during acute intestinal inflammation, and in response to systemic anaphylaxis. Acute inflammation was produced after infection with Nippostrongylus brasiliensis and rats were studied at day 0 (control), day 4 (early), day 10 (acute), and day 35 (postinfection). At the latter stage, immune rats were also studied during anaphylaxis induced by i.v. N. brasiliensis antigen. In each study, blood and urine were sampled over 5 h after the probes were simultaneously injected into ligated loops in anesthetized rats. In controls, small quantities (less than 0.04% and 0.002% of the administered dose for 51Cr-EDTA and ovalbumin, respectively) appeared in the circulation and plateaued at 1 h. During acute inflammation, the appearance of both probes continued to increase with time. Compared with controls, 5-h values for 51Cr-EDTA and ovalbumin were (a) significantly elevated at day 4 (p less than 0.005), (b) increased approximately 20-fold at day 10 (p less than 0.005 and less than 0.01, respectively), and (c) normal at day 35. Urinary recovery of 51Cr-EDTA followed the same pattern. During anaphylaxis, appearance of the probes in the circulationmore » increased at 1 h to values approximately 10-fold those in controls (p less than 0.001 and less than 0.01, for 51Cr-EDTA and ovalbumin, respectively), and then declined. Urinary recovery of 51Cr-EDTA over 5 h was also significantly increased. We conclude that epithelial barrier function becomes impaired during both acute inflammation and anaphylaxis. In this rat model, gut permeability changes to 51Cr-EDTA reflect gut permeability changes to macromolecular antigens.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
McMaster Univ., Hamilton, ON (Canada)
OSTI Identifier:
6969218
Resource Type:
Journal Article
Journal Name:
Gastroenterology; (United States)
Additional Journal Information:
Journal Volume: 94:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EDTA; MEMBRANE TRANSPORT; OVALBUMIN; RATS; IMMUNE REACTIONS; SMALL INTESTINE; INFLAMMATION; PERMEABILITY; ANAPHYLAXIS; BIOLOGICAL MODELS; CHROMIUM 51; COMPARATIVE EVALUATIONS; EPITHELIUM; NIPPOSTRONGYLUS; PARASITIC DISEASES; TRACER TECHNIQUES; AMINO ACIDS; ANIMAL TISSUES; ANIMALS; ASCHELMINTHES; BETA DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CARBOXYLIC ACIDS; CHELATING AGENTS; CHROMIUM ISOTOPES; DIGESTIVE SYSTEM; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; GASTROINTESTINAL TRACT; GLUCOPROTEINS; GLYCOPROTEINS; HELMINTHS; HOOKWORM; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; INTESTINES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NEMATODES; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANS; PATHOLOGICAL CHANGES; PROTEINS; RADIOISOTOPES; RODENTS; SACCHARIDES; SYMPTOMS; TISSUES; VERTEBRATES; 550901* - Pathology- Tracer Techniques

Citation Formats

Ramage, J K, Stanisz, A, Scicchitano, R, Hunt, R H, and Perdue, M H. Effect of immunologic reactions on rat intestinal epithelium. Correlation of increased permeability to chromium 51-labeled ethylenediaminetetraacetic acid and ovalbumin during acute inflammation and anaphylaxis. United States: N. p., 1988. Web.
Ramage, J K, Stanisz, A, Scicchitano, R, Hunt, R H, & Perdue, M H. Effect of immunologic reactions on rat intestinal epithelium. Correlation of increased permeability to chromium 51-labeled ethylenediaminetetraacetic acid and ovalbumin during acute inflammation and anaphylaxis. United States.
Ramage, J K, Stanisz, A, Scicchitano, R, Hunt, R H, and Perdue, M H. 1988. "Effect of immunologic reactions on rat intestinal epithelium. Correlation of increased permeability to chromium 51-labeled ethylenediaminetetraacetic acid and ovalbumin during acute inflammation and anaphylaxis". United States.
@article{osti_6969218,
title = {Effect of immunologic reactions on rat intestinal epithelium. Correlation of increased permeability to chromium 51-labeled ethylenediaminetetraacetic acid and ovalbumin during acute inflammation and anaphylaxis},
author = {Ramage, J K and Stanisz, A and Scicchitano, R and Hunt, R H and Perdue, M H},
abstractNote = {In these studies we compared jejunal permeability to two probes--chromium 51-labeled ethylenediaminetetraacetic acid (51Cr-EDTA) (mol wt, 360) and ovalbumin (mol wt, 45,000)--under control conditions, during acute intestinal inflammation, and in response to systemic anaphylaxis. Acute inflammation was produced after infection with Nippostrongylus brasiliensis and rats were studied at day 0 (control), day 4 (early), day 10 (acute), and day 35 (postinfection). At the latter stage, immune rats were also studied during anaphylaxis induced by i.v. N. brasiliensis antigen. In each study, blood and urine were sampled over 5 h after the probes were simultaneously injected into ligated loops in anesthetized rats. In controls, small quantities (less than 0.04% and 0.002% of the administered dose for 51Cr-EDTA and ovalbumin, respectively) appeared in the circulation and plateaued at 1 h. During acute inflammation, the appearance of both probes continued to increase with time. Compared with controls, 5-h values for 51Cr-EDTA and ovalbumin were (a) significantly elevated at day 4 (p less than 0.005), (b) increased approximately 20-fold at day 10 (p less than 0.005 and less than 0.01, respectively), and (c) normal at day 35. Urinary recovery of 51Cr-EDTA followed the same pattern. During anaphylaxis, appearance of the probes in the circulation increased at 1 h to values approximately 10-fold those in controls (p less than 0.001 and less than 0.01, for 51Cr-EDTA and ovalbumin, respectively), and then declined. Urinary recovery of 51Cr-EDTA over 5 h was also significantly increased. We conclude that epithelial barrier function becomes impaired during both acute inflammation and anaphylaxis. In this rat model, gut permeability changes to 51Cr-EDTA reflect gut permeability changes to macromolecular antigens.},
doi = {},
url = {https://www.osti.gov/biblio/6969218}, journal = {Gastroenterology; (United States)},
number = ,
volume = 94:6,
place = {United States},
year = {Wed Jun 01 00:00:00 EDT 1988},
month = {Wed Jun 01 00:00:00 EDT 1988}
}