Phosphorylation of varicella-zoster virus glycoprotein gpI by mammalian casein kinase II and casein kinase I
- Univ. of Iowa College of Medicine, Iowa City (USA)
- Univ. of California, Riverside (USA)
Varicella-zoster virus (VZV) glycoprotein gpI is the predominant viral glycoprotein within the plasma membranes of infected cells. This viral glycoprotein is phosphorylated on its polypeptide backbone during biosynthesis. In this report, the authors investigated the protein kinases which participate in the phosphorylation events. Under in vivo conditions, VZV gpI was phosphorylated on its serine and threonine residues by protein kinases present within lysates of either VZV-infected or uninfected cells. Because this activity was diminished by heparin, a known inhibitor of casein kinase II, isolated gpI was incubated with purified casein kinase II and shown to be phosphorylated in an in vitro assay containing ({gamma}-{sup 32}P)ATP. The same glycoprotein was phosphorylated when ({sup 32}P)GTP was substituted for ({sup 32}P)ATP in the protein kinase assay. They also tested whether VZV gpI was phosphorylated by two other ubiquitous mammalian protein kinases--casein kinase I and cyclic AMP-dependent kinase--and found that only casein kinase I modified gpI. When the predicted 623-amino-acid sequence of gpI was examined, two phosphorylation sites known to be optimal for casein kinase II were observed. In summary, this study showed that VZV gpI was phosphorylated by each of two mammalian protein kinases (casein kinase I and casein kinase II) and that potential serine-threonine phosphorylation sites for each of these two kinases were present in the viral glycoprotein.
- OSTI ID:
- 6968843
- Journal Information:
- Journal of Virology; (USA), Vol. 63:9; ISSN 0022-538X
- Country of Publication:
- United States
- Language:
- English
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GLYCOPROTEINS
PHOSPHORYLATION
MEASLES VIRUS
ENZYME ACTIVITY
PHOSPHOTRANSFERASES
BIOCHEMISTRY
AMINO ACID SEQUENCE
ATP
CASEIN
PHOSPHORUS 32
SERINE
THREONINE
AMINO ACIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ENZYMES
HYDROXY ACIDS
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
MOLECULAR STRUCTURE
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PARASITES
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIOISOTOPES
TRANSFERASES
VIRUSES
550201* - Biochemistry- Tracer Techniques