Flow cytometric measurement of pollutant stresses on algal cells

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Cytometry (Baltimore); (United States)

DOI:https://doi.org/10.1002/cyto.990090209· OSTI ID:6968510

Berglund, D L; Eversman, S

The lichen Usnea fulvoreagens (Raes). Raes. was treated with four pH levels (5.5, 4.5, 3.5, and 2.5) of simulated acid rain (sulfuric acid, nitric acid, and a 1:1 combination of both) and automobile exhaust. The samples were dissociated and analyzed by a Becton-Dickinson FACS 440 flow cytometer. Analyses included measurement of chlorophyll autofluorescence and fluorescence due to uptake of fluorescein diacetate (FDA) and calcofluor white M2R (CFW). Cell parameters measured were esterase activity (FDA), membrane permeability (FDA, CFW), and intracellular pH (FDA). Mean fluorescence intensity from FDA staining and numbers of events were incorporated with autofluorescence information to produce a stress index of relative cell stress. Results indicated that highly stressed samples (lower pH treatments and greater exposure to exhaust) exhibited a low stress index of FDA fluorescence.Au

• Research Organization: Montana State Univ., Bozeman (USA)
• OSTI ID: 6968510
• Journal Information: Cytometry (Baltimore); (United States), Vol. 9:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID RAIN
TOXICITY
AIR POLLUTION
BIOLOGICAL INDICATORS
ESTERASES
ENZYME ACTIVITY
EXHAUST GASES
LICHENS
BIOLOGICAL STRESS
AUTOMOBILES
CELL FLOW SYSTEMS
CELL MEMBRANES
FLUORESCENCE
PERMEABILITY
SIMULATION
ALGAE
ATMOSPHERIC PRECIPITATIONS
CELL CONSTITUENTS
ENZYMES
FLUIDS
FUNGI
GASEOUS WASTES
GASES
HYDROLASES
LUMINESCENCE
MEMBRANES
PLANTS
POLLUTION
RAIN
VEHICLES
WASTES
560300* - Chemicals Metabolism & Toxicology