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Title: Studies of the regulation of nitrate influx by barley seedlings using sup 13 NO sub 3 sup minus 1. [Hordeum vulgare L]

Abstract

Using {sup 13}NO{sub 3}{sup {minus}}, effects of various NO{sub 3}{sup {minus}} pretreatments upon NO{sub 3}{sup {minus}} influx were studied in intact roots of barley (Hordeum vulgare L. cv Klondike). Prior exposure of roots to NO{sub 3}{sup {minus}} increased NO{sub 3}{sup {minus}} influx and net NO{sub 3}{sup {minus}} uptake. This induction of NO{sub 3}{sup {minus}} uptake was dependent both on time and external NO{sub 3}{sup {minus}} concentration ((NO{sub 3}{sup {minus}})). During induction influx was positively correlated with root (NO{sub 3}{sup {minus}}). In the postinduction period, however, NO{sub 3}{sup {minus}} influx declined as root (NO{sub 3}{sup {minus}}) increased. It is suggested that induction and negative feedback regulation are independent processes: induction appears to depend upon some critical cytoplasmic (NO{sub 3}{sup {minus}}); removal of external NO{sub 3}{sup {minus}} caused a reduction of {sup 13}NO{sub 3}{sup {minus}} influx even though mean root (NO{sub 3}{sup {minus}}) remained high. It is proposed that cytoplasmic (NO{sub 3}{sup {minus}}) is depleted rapidly under these conditions resulting in deinduction of the NO{sub 3}{sup {minus}} transport system. Beyond 50 micromoles per gram (NO{sub 3}{sup {minus}}), {sup 13}NO{sub 3}{sup {minus}} influx was negatively correlated with root (NO{sub 3}{sup {minus}}). However, it is unclear whether root (NO{sub 3}{sup {minus}}) per se ormore » some product(s) of NO{sub 3}{sup {minus}} assimilation are responsible for the negative feedback effects.« less

Authors:
; ; ;
Publication Date:
OSTI Identifier:
6968331
Resource Type:
Journal Article
Journal Name:
Plant Physiology; (USA)
Additional Journal Information:
Journal Volume: 90:3; Journal ID: ISSN 0032-0889
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BARLEY; METABOLISM; NITRATES; UPTAKE; NITROGEN 13; NITROGEN COMPOUNDS; ROOTS; SEEDLINGS; TRACER TECHNIQUES; BETA DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; CEREALS; ELECTRON CAPTURE RADIOISOTOPES; GRASS; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; LILIOPSIDA; MAGNOLIOPHYTA; MINUTES LIVING RADIOISOTOPES; NITROGEN ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; OXYGEN COMPOUNDS; PLANTS; RADIOISOTOPES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Siddiqi, M Y, Glass, A D.M., Ruth, T J, and Fernando, M. Studies of the regulation of nitrate influx by barley seedlings using sup 13 NO sub 3 sup minus 1. [Hordeum vulgare L]. United States: N. p., 1989. Web. doi:10.1104/pp.90.3.806.
Siddiqi, M Y, Glass, A D.M., Ruth, T J, & Fernando, M. Studies of the regulation of nitrate influx by barley seedlings using sup 13 NO sub 3 sup minus 1. [Hordeum vulgare L]. United States. https://doi.org/10.1104/pp.90.3.806
Siddiqi, M Y, Glass, A D.M., Ruth, T J, and Fernando, M. 1989. "Studies of the regulation of nitrate influx by barley seedlings using sup 13 NO sub 3 sup minus 1. [Hordeum vulgare L]". United States. https://doi.org/10.1104/pp.90.3.806.
@article{osti_6968331,
title = {Studies of the regulation of nitrate influx by barley seedlings using sup 13 NO sub 3 sup minus 1. [Hordeum vulgare L]},
author = {Siddiqi, M Y and Glass, A D.M. and Ruth, T J and Fernando, M},
abstractNote = {Using {sup 13}NO{sub 3}{sup {minus}}, effects of various NO{sub 3}{sup {minus}} pretreatments upon NO{sub 3}{sup {minus}} influx were studied in intact roots of barley (Hordeum vulgare L. cv Klondike). Prior exposure of roots to NO{sub 3}{sup {minus}} increased NO{sub 3}{sup {minus}} influx and net NO{sub 3}{sup {minus}} uptake. This induction of NO{sub 3}{sup {minus}} uptake was dependent both on time and external NO{sub 3}{sup {minus}} concentration ((NO{sub 3}{sup {minus}})). During induction influx was positively correlated with root (NO{sub 3}{sup {minus}}). In the postinduction period, however, NO{sub 3}{sup {minus}} influx declined as root (NO{sub 3}{sup {minus}}) increased. It is suggested that induction and negative feedback regulation are independent processes: induction appears to depend upon some critical cytoplasmic (NO{sub 3}{sup {minus}}); removal of external NO{sub 3}{sup {minus}} caused a reduction of {sup 13}NO{sub 3}{sup {minus}} influx even though mean root (NO{sub 3}{sup {minus}}) remained high. It is proposed that cytoplasmic (NO{sub 3}{sup {minus}}) is depleted rapidly under these conditions resulting in deinduction of the NO{sub 3}{sup {minus}} transport system. Beyond 50 micromoles per gram (NO{sub 3}{sup {minus}}), {sup 13}NO{sub 3}{sup {minus}} influx was negatively correlated with root (NO{sub 3}{sup {minus}}). However, it is unclear whether root (NO{sub 3}{sup {minus}}) per se or some product(s) of NO{sub 3}{sup {minus}} assimilation are responsible for the negative feedback effects.},
doi = {10.1104/pp.90.3.806},
url = {https://www.osti.gov/biblio/6968331}, journal = {Plant Physiology; (USA)},
issn = {0032-0889},
number = ,
volume = 90:3,
place = {United States},
year = {Sat Jul 01 00:00:00 EDT 1989},
month = {Sat Jul 01 00:00:00 EDT 1989}
}