Effects of lipopolysaccharide on the catabolic activity of macrophages
The ability of macrophages to degrade and catabolize antigens is of relevance both as a means to process complex antigens prior to presentation to T cells, as well as a way to down regulate immune responses by destroying the antigenicity of polypeptides. With these considerations, the authors have investigated the regulation of macrophage catabolic activity by lipopolysaccharide (LPS). Catabolic activity was quantitated by following the distribution and molecular form of /sup 125/-I labelled surface components of heat-killed Listeria monocytogenes (HKLM) subsequent to their uptake by macrophages. They have compared the catabolic activity of macrophages from peritoneal exudates of mice injected i.p. with saline or LPS and have found that LPS-elicited macrophages display a greatly enhanced (3 fold) rate of catabolism. This increase in catabolic activity peaks 3 days after LPS injection and steadily declines thereafter, approaching a baseline level after 3 weeks. The enhancement of catabolic activity is under LPS gene control. LPS-elicited macrophages rapidly destroy the antigenicity of bacterial antigens and function poorly as antigen presenting cells in vitro. These results suggest that LPS elicits a macrophage population specialized for antigen degradation functions with negative regulatory effects on the induction of specific immune responses.
- Research Organization:
- Emory Univ., Atlanta, GA
- OSTI ID:
- 6965909
- Report Number(s):
- CONF-8604222-; TRN: 87-005743
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:4; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
LIPOPOLYSACCHARIDES
BIOLOGICAL EFFECTS
MACROPHAGES
BIOLOGICAL FUNCTIONS
ANTIGENS
BACTERIA
CATABOLISM
IMMUNE REACTIONS
IODINE 125
TRACER TECHNIQUES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
CARBOHYDRATES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FUNCTIONS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIPIDS
METABOLISM
MICROORGANISMS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHAGOCYTES
POLYSACCHARIDES
RADIOISOTOPES
SACCHARIDES
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques