Effect of hypolipidemic drugs on basal and stimulated adenylate cyclase activity in tumor cells

Journal Article · Sat Feb 01 00:00:00 EST 1986 · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)

OSTI ID:6958313

Bershtein, L M; Kovaleva, I G; Rozenberg, O A

This paper studies adenylate cyclase acticvity in Ehrlich's ascites carcinoma (EAC) cells during administration of drugs with a hypolipidemic action. Seven to eight days before they were killed, male mice ingested the antidiabetic biguanide phenformin, and the phospholipid-containing preparation Essentiale in drinking water. The cAMP formed was isolated by chromatography on Silufol plates after incubation of the enzyme preparation with tritium-ATP, or was determined by the competitive binding method with protein. It is shown that despite the possible differences in the concrete mechanism of action of the hypolipidemic agents chosen for study on the cyclase system, the use of such agents, offers definite prospects for oriented modification of the hormone sensitivity of tumor cells.

• Research Organization: Ministry of Health of The USSR, Leningrad
• OSTI ID: 6958313
• Journal Information: Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Vol. 100:9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ASCITES TUMOR CELLS
SENSITIVITY
CYCLASES
ENZYME ACTIVITY
DRUGS
BIOLOGICAL EFFECTS
RADIORECEPTOR ASSAY
AMP
ATP
EHRLICH ASCITES TUMOR
HORMONES
PHOSPHOLIPIDS
TRANSPLANTS
TRITIUM COMPOUNDS
ANIMAL CELLS
ENZYMES
ESTERS
EXPERIMENTAL NEOPLASMS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LYASES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
TRACER TECHNIQUES
TUMOR CELLS
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques