Characterization of the action of epidermal growth factor in three different biological systems
In general, these studies characterized in each system one or more of the following aspects of EGF's biological action: EGF binding, EGF-induced phosphorylation, or EGF-stimulated DNA synthesis. In human corneal fibroblasts (HCF), EGF binds to specific, saturable, high affinity receptors and stimulated (/sup 3/H)-thymidine incorporation, and in vitro measurement of DNA synthesis, approximately 2.5 fold above control cultures, with maximal stimulation occurring at approximately 1 nM EGF. In addition, fibroblasts growth factor (FGF) and insulin stimulated maximal (/sup 3/H)-thymidine incorporation in HCF at approximately 100 ng ml/sup -1/ and 1 nM, respectively. In combination with dexamethasone (5 nM), the stimulation of (/sup 3/H)-thymidine incorporation by EGF was maintained. However, dexamethasone abolished the stimulatory action of FGF. Highly purified preparations of human placental outer cell membranes and intracellular organelles were assayed for functional EGF binding proteins (EGF-receptors). Functionality of a protein was determined by covalent labeling with (/sup 125/I)-EGF and EGF-stimulated autophosphorylation.
- Research Organization:
- Louisville Univ., KY (USA)
- OSTI ID:
- 6956515
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
RECEPTORS
PROTEINS
DNA REPLICATION
PHOSPHORYLATION
CELL MEMBRANES
CORNEA
DEXAMETHASONE
EPIDERMIS
FIBROBLASTS
MAN
PLACENTA
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ADRENAL HORMONES
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
AZINES
BODY
BODY AREAS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONNECTIVE TISSUE CELLS
CORTICOSTEROIDS
EPITHELIUM
EYES
FACE
FETAL MEMBRANES
GLUCOCORTICOIDS
HEAD
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
NUCLEIC ACID REPLICATION
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PREGNANES
PRIMATES
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SENSE ORGANS
SKIN
SOMATIC CELLS
STEROIDS
TISSUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques